School of Science and Technology, Kwansei Gakuin University, Sanda, Japan.
Institute of Analytical Chemistry and Radiochemistry, CCB-Center for Chemistry and Biomedicine, Leopold-Franzens University, Innsbruck, Austria.
J Biophotonics. 2018 Apr;11(4):e201700115. doi: 10.1002/jbio.201700115. Epub 2017 Dec 18.
In this study, the distribution of biomaterials and its molecular mechanism of embryonic development in Japanese medaka fish were analyzed nondestructively and noninvasively without staining using near-infrared (NIR) imaging. The microscopic NIR imaging system used in this research was a device capable of ultra-high-speed imaging; using this system, one can acquire microscopic imaging data in a few seconds. Therefore, the medaka eggs remained alive throughout measurements and were successfully monitored in vivo. The distributions of biomolecules were examined by mapping the intensities of NIR bands resulting from lipids, proteins and water in 2 dimensions (2D). The structures of eyes, lipid bilayer membranes, micelles and water-structure differences at the interface of different substances constituting different structures on the egg were visualized. Furthermore, insights on the metabolic mechanisms of lipids and membrane functions were drawn from the biased distribution of lipoproteins and the presence of unsaturated fatty acids in the egg membrane. These results indicated the potential for NIR imaging in evaluating the biological functions and metabolic systems of cells and embryos.
在这项研究中，我们使用近红外（NIR）成像技术，对日本青鳉鱼胚胎发育过程中的生物材料分布及其分子机制进行了非破坏性、非侵入性、无需染色的分析。本研究中使用的微观 NIR 成像系统是一种能够进行超高速成像的设备；使用该系统，可以在几秒钟内获取微观成像数据。因此，青鳉鱼卵在整个测量过程中保持存活，并成功地进行了体内监测。通过对脂质、蛋白质和水的 NIR 波段强度进行二维（2D）绘图，对生物分子的分布进行了检查。可视化了眼睛、脂质双层膜、胶束以及不同物质界面处的水结构差异等结构。此外，从脂蛋白的偏置分布和卵膜中不饱和脂肪酸的存在中，我们得出了关于脂质代谢机制和膜功能的见解。这些结果表明，NIR 成像在评估细胞和胚胎的生物功能和代谢系统方面具有潜力。
