评估两种用于检测奶牛亚临床酮病的现场检测方法。
Evaluation of two cowside tests for the detection of subclinical ketosis in dairy cows.
作者信息
Nielen M, Aarts M G, Jonkers A G, Wensing T, Schukken Y H
机构信息
Department of Herd Health and Reproduction, Utrecht University, The Netherlands.
出版信息
Can Vet J. 1994 Apr;35(4):229-32.
The goal of this study was to evaluate the sensitivity and specificity of two cowside tests for subclinical ketosis in dairy cows. The tests utilize milk and urine samples, respectively. One hundred and eighty-five cows, one to sixty days postpartum, were sampled for milk, urine, and blood. Subclinical ketosis was defined with serum beta-hydroxybutyrate measurements. The sensitivity and the specificity of both tests at different beta-hydroxybutyrate levels were estimated. When subclinical ketosis was defined at beta-hydroxybutyrate levels of 1.4 mmol/L and higher, the milk test had sensitivity of 90% and specificity of 96%. The urine test lacked specificity (values < 67%), but sensitivity was 100% at beta-hydroxybutyrate levels of 1.4 mmol/L upward. Both the milk and urine test can be used to monitor subclinical ketosis in a herd. Milk testing is preferred, because of the easy obtainability of milk combined with the overall better test characteristics.
本研究的目的是评估两种奶牛现场检测方法对奶牛亚临床酮病的敏感性和特异性。这两种检测方法分别使用牛奶和尿液样本。对185头产后1至60天的奶牛采集了牛奶、尿液和血液样本。亚临床酮病通过血清β-羟基丁酸酯测量来定义。估计了两种检测方法在不同β-羟基丁酸酯水平下的敏感性和特异性。当亚临床酮病定义为β-羟基丁酸酯水平在1.4 mmol/L及以上时，牛奶检测的敏感性为90%，特异性为96%。尿液检测缺乏特异性（值<67%），但在β-羟基丁酸酯水平为1.4 mmol/L及以上时，敏感性为100%。牛奶和尿液检测均可用于监测牛群中的亚临床酮病。由于牛奶易于获取且检测特性总体更好，因此更倾向于使用牛奶检测。
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