Ciric Andrija, Jelikic-Stankov Milena, Cvijovic Milica, Djurdjevic Predrag
University of Kragujevac, Faculty of Science, Kragujevac, Serbia.
University of Belgrade, Faculty of Pharmacy, Belgrade, Serbia.
Biomed Chromatogr. 2018 Apr;32(4). doi: 10.1002/bmc.4150. Epub 2017 Dec 15.
An isocratic RP-HPLC method for the separation and identification of selected flavonoids (quercetin, rutin, luteolin-7-O-glucoside, kaempferol and kaempferol-3-O-glucoside) in commercial berry juices (blackcurrant, blueberry, red raspberry and cherry) was developed with the aid of central composite design and response surface methodology. The optimal separation conditions were a mobile phase of 85:15 (% v/v) water-acetonitrile, pH 2.8 (adjusted with formic acid), flow rate 0.5 mL min and column temperature 35°C. The obtained levels of bioflavonoids (mg per 100 mL of juice) were as follows: for quercetin, ca. 0.21-5.12; for kaempferol, ca. 0.05-1.2; for rutin, ca. 0.4-6.5; for luteolin-7-O-glucoside, ca. 5.6-10.2; and for kaempferol-3-O-glucoside, ca. 0.02-0.12. These are considerably lower than the values in fresh fruits. Total phenolic, flavonoid and anthocyanin contents were determined spectrophotometrically. Total flavonoid content varied as follows: blackcurrant > blueberry > red raspberry > cherry. The antioxidant activity of juice extracts (DPPH and ABTS methods) expressed as IC values varied from 8.56 to 14.05 mg L . These values are ~2.5-3 times lower than quercetin, ascorbic acid and Trolox®, but compared with rutin and butylhydroxytoluene, berries show similar or better antioxidant activity by both the DPPH and ABTS methods.
借助中心复合设计和响应面法，开发了一种等度反相高效液相色谱法，用于分离和鉴定市售浆果果汁（黑加仑、蓝莓、红树莓和樱桃）中选定的黄酮类化合物（槲皮素、芦丁、木犀草素 -7-O-葡萄糖苷、山奈酚和山奈酚 -3-O-葡萄糖苷）。最佳分离条件为流动相为85:15（% v/v）水 - 乙腈，pH值2.8（用甲酸调节），流速0.5 mL/min，柱温35°C。所获得的生物类黄酮水平（每100 mL果汁中的毫克数）如下：槲皮素约为0.21 - 5.12；山奈酚约为0.05 - 1.2；芦丁约为0.4 - 6.5；木犀草素 -7-O-葡萄糖苷约为5.6 - 10.2；山奈酚 -3-O-葡萄糖苷约为0.02 - 0.12。这些值远低于新鲜水果中的值。通过分光光度法测定了总酚、黄酮和花青素含量。总黄酮含量变化如下：黑加仑＞蓝莓＞红树莓＞樱桃。以IC值表示的果汁提取物的抗氧化活性（DPPH和ABTS法）在8.56至14.05 mg/L之间变化。这些值比槲皮素、抗坏血酸和Trolox®低约2.5 - 3倍，但与芦丁和丁基羟基甲苯相比，通过DPPH和ABTS法，浆果显示出相似或更好的抗氧化活性。
