Institute of Biostructures and Bioimaging, CNR, Naples, Italy.
Dipartimento di Agraria, Università degli Studi di Napoli Federico II, Portici, NA, Italy.
Protein Sci. 2018 Mar;27(3):620-632. doi: 10.1002/pro.3348. Epub 2017 Dec 5.
Prosystemin, originally isolated from Lycopersicon esculentum, is a tomato pro-hormone of 200 aminoacid residues which releases a bioactive peptide of 18 aminoacids called Systemin. This signaling peptide is involved in the activation of defense genes in solanaceous plants in response to herbivore feeding damage. Using biochemical, biophysical and bioinformatics approaches we characterized Prosystemin, showing that it is an intrinsically disordered protein possessing a few secondary structure elements within the sequence. Plant treatment with recombinant Prosystemin promotes early and late plant defense genes, which limit the development and survival of Spodoptera littoralis larvae fed with treated plants.
Prosystemin，最初从番茄中分离得到，是一个 200 个氨基酸残基的番茄前激素，它释放出一个 18 个氨基酸的生物活性肽，称为 Systemin。这种信号肽参与了茄科植物在受到草食动物取食损伤后的防御基因的激活。我们使用生物化学、生物物理和生物信息学方法对 Prosystemin 进行了表征，表明它是一种固有无序的蛋白质，在序列中具有一些二级结构元件。用重组 Prosystemin 处理植物可促进早期和晚期植物防御基因的表达，从而限制取食处理植物的斜纹夜蛾幼虫的发育和存活。
