a Systemomics Center, College of Pharmacy, and Genomics Research Center (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China) , Harbin Medical University , Harbin , Heilongjiang Province , China.
b HMU-UCFM Centre for Infection and Genomics , Harbin Medical University , Harbin , Heilongjiang Province , China.
Cancer Biol Ther. 2017 Dec 2;18(12):990-999. doi: 10.1080/15384047.2017.1394542. Epub 2017 Nov 27.
Ovarian cancer is the third most common cancer in the female reproductive organs and epithelial ovarian cancer has the highest lethality of all gynecological cancers. Pomegranate fruit juice (PFJ) has been shown to inhibit the growth of several types of cancer other than ovarian cancer. In this study, we exposed the ovarian cancer cell line A2780 to PFJ and two of its components (ellagic acid and luteolin). MTT and wound healing assays demonstrated that all three treatments suppressed the proliferation and migration of the ovarian cancer cells. In addition, western blotting and ELISA assays showed that the expression levels of MMP2 and MMP9 gradually decreased after treatment with increasing concentrations of ellagic acid and luteolin. To confirm our findings in the in vitro experiments, we used another ovarian cancer cell line, ES-2, in nude mice experiments. All three treatments inhibited tumor growth without obvious side-effects. Furthermore, compared with the control group, the expression levels of MMP2 and MMP9 were depressed. Ellagic acid induced a greater effect than luteolin, suggesting that ellagic acid might be a promising candidate for further preclinical testing for treatment of human ovarian cancer.
卵巢癌是女性生殖器官中第三常见的癌症，而上皮性卵巢癌是所有妇科癌症中致死率最高的。石榴汁（PFJ）已被证明可以抑制除卵巢癌以外的几种癌症的生长。在这项研究中，我们将卵巢癌细胞系 A2780 暴露于 PFJ 及其两种成分（鞣花酸和木樨草素）中。MTT 和划痕愈合实验表明，三种处理均抑制了卵巢癌细胞的增殖和迁移。此外，Western blot 和 ELISA 检测表明，随着鞣花酸和木樨草素浓度的增加，MMP2 和 MMP9 的表达水平逐渐降低。为了证实我们在体外实验中的发现，我们在裸鼠实验中使用了另一种卵巢癌细胞系 ES-2。三种处理均抑制了肿瘤生长，没有明显的副作用。此外，与对照组相比，MMP2 和 MMP9 的表达水平降低。鞣花酸的作用大于木樨草素，表明鞣花酸可能是进一步进行临床前测试以治疗人类卵巢癌的有前途的候选药物。
Zotero 插件
