Gürsoy Elif Naz, Balabanli Kanuni Barbaros, Tuğcu Demiröz Fatma Nur, Coşkun Cevher Şule
Department of Biology, Faculty of Science, Gazi University, Ankara, Turkiye.
Pharmaceutical Technology, Faculty of Pharmacy, Gazi University, Ankara, Turkiye.
Turk J Biol. 2024 Oct 22;48(6):364-378. doi: 10.55730/1300-0152.2712. eCollection 2024.
BACKGROUND/AIM: The hyperglycemic environment in diabetes disrupts normal wound-healing processes, leading to chronic wounds. This study investigated whether the combination of the phenolic compounds ellagic acid and carnosic acid shows synergistic effects on diabetic wound healing and oxidative parameters in diabetic rats.
Diabetic rats were divided into control, untreated, Carbopol 974P treated, topical treatment, and oral gavage treatment groups. Ellagic acid and carnosic acid in combination were applied topically and as oral gavage to the full-thickness excisional wounds of all animals except for those in the control group. We investigated oxidative events with malondialdehyde, glutathione, nitric oxide, protein carbonyl, collagen spectrophotometrically, and matrix metalloproteinase 2 and 9 and advanced oxidation protein product levels using ELISA.
The combination of ellagic acid and carnosic acid decreased malondialdehyde, nitric oxide, protein carbonyl, advanced oxidation protein product, and metalloproteinase 9 levels and increased the rate of wound contraction and collagen levels in the diabetic wound healing model.
The combined use of ellagic acid and carnosic acid showed a synergistic effect, enhanced wound healing, and decreased oxidative stress. This combination may provide effective therapy for chronic nonhealing wounds that occur as a complication of diabetes.
背景/目的：糖尿病中的高血糖环境会破坏正常的伤口愈合过程，导致慢性伤口。本研究调查了酚类化合物鞣花酸和肌醇六磷酸联合使用是否对糖尿病大鼠的伤口愈合和氧化参数具有协同作用。
将糖尿病大鼠分为对照组、未治疗组、卡波姆974P治疗组、局部治疗组和灌胃治疗组。除对照组动物外，对所有动物的全层切除伤口局部应用并灌胃给予鞣花酸和肌醇六磷酸的组合。我们通过分光光度法检测丙二醛、谷胱甘肽、一氧化氮、蛋白质羰基、胶原蛋白，并使用酶联免疫吸附测定法检测基质金属蛋白酶2和9以及晚期氧化蛋白产物水平，以研究氧化事件。
在糖尿病伤口愈合模型中，鞣花酸和肌醇六磷酸的组合降低了丙二醛、一氧化氮、蛋白质羰基、晚期氧化蛋白产物和金属蛋白酶9的水平，并提高了伤口收缩率和胶原蛋白水平。
鞣花酸和肌醇六磷酸联合使用具有协同作用，可促进伤口愈合并降低氧化应激。这种组合可能为作为糖尿病并发症出现的慢性不愈合伤口提供有效的治疗方法。
