视网膜母细胞瘤细胞系（Y79）中双微小染色体和均匀染色区的同时存在以及均质染色区处N-myc的扩增

Simultaneous existence of double minute chromosomes and a homogeneously staining region in a retinoblastoma cell line (Y79) and amplification of N-myc at HSR.

作者信息

Inazawa J, Abe T, Inoue K, Nishigaki H, Horiike S, Taniwaki M, Misawa S, Takino T

机构信息

Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.

出版信息

Cancer Genet Cytogenet. 1989 Jan;37(1):133-7. doi: 10.1016/0165-4608(89)90082-4.

DOI:10.1016/0165-4608(89)90082-4

PMID:2917328

Abstract

We observed double minute chromosomes (dmin) and a homogeneously staining region (HSR) in the same metaphase cells obtained from a retinoblastoma cell line, Y79. All of the 132 metaphases examined contained an HSR on the short arm of chromosome 1(1pHSR) and five cells (3.8%) had two to four dmin. To determine whether 1pHSR and dmin carried amplified N-myc sequences, we performed an in situ hybridization using an N-myc probe. Silver grains clustered on and along the 1pHSR, but not on the dmin. These findings indicate that the HSR on chromosome 1 is associated with amplification of N-myc in Y79 cells.

摘要

我们在源自视网膜母细胞瘤细胞系Y79的同一中期细胞中观察到双微体染色体（dmin）和均匀染色区（HSR）。在检查的132个中期相中，所有细胞在1号染色体短臂上均含有一个HSR（1pHSR），5个细胞（3.8%）有2至4个dmin。为了确定1pHSR和dmin是否携带扩增的N-myc序列，我们使用N-myc探针进行了原位杂交。银颗粒聚集在1pHSR上及其周围，但不在dmin上。这些发现表明，1号染色体上的HSR与Y79细胞中N-myc的扩增有关。