Yang Fen, Xie Shaowen, Liu Jinxin, Wei Chaoyang, Zhang Hongzhi, Chen Tao, Zhang Jing
Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China; Key Laboratory of Land Surface Pattern and Simulation, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China; Key Laboratory of Land Surface Pattern and Simulation, Chinese Academy of Sciences, Beijing, 100101, China.
Chemosphere. 2018 Feb;193:777-784. doi: 10.1016/j.chemosphere.2017.11.098. Epub 2017 Nov 20.
Birds are at a higher level in the food chain; however, the potential bioaccumulation and biotransformation of arsenic (As) in birds in As mines has rarely been studied. In this study, four passerine bird species (tree sparrow [Passer montanus], light-vented bulbul [Pycnonotus sinensis], Garrulax canorus [Leucodioptron canorus], and magpie [Pica pica]) were collected from an abandoned As mine in China. The highest recorded As concentrations were 4.95 mg/kg and 51.65 mg/kg in muscles and feathers, respectively. Detection using high-performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC-ICP-MS) revealed six As species, including arsenite (As(III)), arsenate (As(V)), dimethylarsinic acid (DMA), monomethylarsonic acid (MMA), arsenobetaine (AsB) and arsenocholine (AsC), with the former three species as the dominant (>92%) and the latter three as the minor As species (<6.17%). Further analysis of the selected bird samples using the X-ray absorption near edge structure (XANES) technique revealed the existence of As(III)-tris-glutathione (As(III)-GSH), which can be regarded as equivalent to the non-extractable and unidentified As form in the HPLC-ICP-MS data. Both methods revealed similar patterns of As species in the birds from the As mine, with muscles containing mainly inorganic As and DMA and feathers containing mainly inorganic As. The results of this study contribute to the knowledge regarding As accumulation and speciation in terrestrial organisms.
鸟类处于食物链的较高层级；然而，砷矿中鸟类体内砷（As）的潜在生物累积和生物转化鲜有研究。在本研究中，从中国一座废弃的砷矿采集了四种雀形目鸟类（树麻雀[Passer montanus]、淡眉柳莺[Pycnonotus sinensis]、画眉[Garrulax canorus（Leucodioptron canorus）]和喜鹊[Pica pica]）。肌肉和羽毛中记录到的最高砷浓度分别为4.95毫克/千克和51.65毫克/千克。使用高效液相色谱 - 电感耦合等离子体质谱法（HPLC - ICP - MS）检测发现六种砷形态，包括亚砷酸盐（As(III)）、砷酸盐（As(V)）、二甲基砷酸（DMA）、一甲基砷酸（MMA）、砷甜菜碱（AsB）和砷胆碱（AsC），前三种形态为主要形态（>92%），后三种为次要砷形态（<6.17%）。使用X射线吸收近边结构（XANES）技术对所选鸟类样本进行进一步分析，发现存在As(III) - 三谷胱甘肽（As(III) - GSH），其可被视为等同于HPLC - ICP - MS数据中不可提取且未鉴定的砷形态。两种方法都揭示了来自砷矿的鸟类体内砷形态的相似模式，肌肉中主要含有无机砷和DMA，羽毛中主要含有无机砷。本研究结果有助于增进对陆地生物中砷积累和形态的认识。
