Department of Microbiology NYU Langone Medical Center, New York, NY, USA.
Department of Pathology NYU Langone Medical Center, New York, NY, USA.
Nat Microbiol. 2018 Feb;3(2):234-242. doi: 10.1038/s41564-017-0075-5. Epub 2017 Nov 27.
Antibiotic exposure in children has been associated with the risk of inflammatory bowel disease (IBD). Antibiotic use in children or in their pregnant mother can affect how the intestinal microbiome develops, so we asked whether the transfer of an antibiotic-perturbed microbiota from mothers to their children could affect their risk of developing IBD. Here we demonstrate that germ-free adult pregnant mice inoculated with a gut microbial community shaped by antibiotic exposure transmitted their perturbed microbiota to their offspring with high fidelity. Without any direct or continued exposure to antibiotics, this dysbiotic microbiota in the offspring remained distinct from controls for at least 21 weeks. By using both IL-10-deficient and wild-type mothers, we showed that both inoculum and genotype shape microbiota populations in the offspring. Because IL10 mice are genetically susceptible to colitis, we could assess the risk due to maternal transmission of an antibiotic-perturbed microbiota. We found that the IL10 offspring that had received the perturbed gut microbiota developed markedly increased colitis. Taken together, our findings indicate that antibiotic exposure shaping the maternal gut microbiota has effects that extend to the offspring, with both ecological and long-term disease consequences.
儿童时期接触抗生素与炎症性肠病 (IBD) 的风险有关。儿童或其孕妇母亲使用抗生素会影响肠道微生物组的发育方式，因此我们想知道，来自母亲的抗生素扰乱的微生物组转移到她们的孩子身上是否会影响他们患上 IBD 的风险。在这里，我们证明了接受过抗生素暴露塑造的肠道微生物群落接种的无菌成年孕妇老鼠能够将其扰乱的微生物组以高保真度传递给它们的后代。没有任何直接或持续的抗生素暴露，这些后代的失调微生物组与对照组至少保持 21 周的区别。通过使用 IL-10 缺陷型和野生型母亲，我们表明接种物和基因型都会影响后代中的微生物种群。由于 IL10 老鼠在遗传上容易患结肠炎，我们可以评估由于母亲传递抗生素扰乱的微生物组而导致的风险。我们发现，接受了扰乱肠道微生物组的 IL10 后代明显发展出了更严重的结肠炎。总之，我们的研究结果表明，塑造母体肠道微生物组的抗生素暴露会产生影响，既有生态影响，也有长期疾病后果。
