Zeng Deliang, Zhang Xingdi, Wang Xiao, Cao Lingyan, Zheng Ao, Du Jiahui, Li Yongsheng, Huang Qingfeng, Jiang Xinquan
Department of Prosthodontics, School of Medicine, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Oral Bioengineering Laboratory, Shanghai Research Institute of Stomatology, School of Medicine, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Int J Nanomedicine. 2017 Nov 15;12:8277-8287. doi: 10.2147/IJN.S144528. eCollection 2017.
Our previous study revealed that mesoporous Ca-Si-based materials exhibited excellent osteoconduction because dissolved ions could form a layer of hydroxycarbonate apatite on the surface of the materials. However, the biological mechanisms underlying bone regeneration were largely unknown. The main aim of this study was to evaluate the osteogenic ability of large-pore mesoporous Ca-Si-based bioceramics (LPMSCs) by alkaline phosphatase assay, real-time PCR analysis, von Kossa, and alizarin red assay. Compared with large-pore mesoporous silica (LPMS), LPMSCs had a better effect on the osteogenic differentiation of dental pulp cells. LPMSC-2 and LPMSC-3 with higher calcium possessed better osteogenic abilities than LPMSC-1, which may be related to the calcium-sensing receptor pathway. Furthermore, the loading capacity for recombinant human platelet-derived growth factor-BB was satisfactory in LPMSCs. In vivo, the areas of new bone formation in the calvarial defect repair were increased in the LPMSC-2 and LPMSC-3 groups compared with the LPMSC-1 and LPMS groups. We concluded that LPMSC-2 and LPMSC-3 possessed both excellent osteogenic abilities and satisfactory loading capacities, which may be attributed to their moderate Ca/Si molar ratio. Therefore, LPMSCs with moderate Ca/Si molar ratio might be potential alterative grafts for craniomaxillofacial bone regeneration.
我们之前的研究表明，介孔钙硅基材料具有出色的骨传导性，因为溶解的离子可在材料表面形成一层羟基碳酸磷灰石。然而，骨再生背后的生物学机制在很大程度上尚不清楚。本研究的主要目的是通过碱性磷酸酶测定、实时聚合酶链反应分析、冯·科萨染色和茜素红染色试验，评估大孔介孔钙硅基生物陶瓷（LPMSCs）的成骨能力。与大孔介孔二氧化硅（LPMS）相比，LPMSCs对牙髓细胞的成骨分化有更好的效果。钙含量较高的LPMSC-2和LPMSC-3比LPMSC-1具有更好的成骨能力，这可能与钙敏感受体途径有关。此外，LPMSCs对重组人血小板衍生生长因子-BB的负载能力令人满意。在体内，与LPMSC-1组和LPMS组相比，LPMSC-2组和LPMSC-3组颅骨缺损修复中新骨形成的面积增加。我们得出结论，LPMSC-2和LPMSC-3既具有出色的成骨能力又具有令人满意的负载能力，这可能归因于它们适度的钙硅摩尔比。因此钙硅摩尔比适度的LPMSCs可能是颅颌面骨再生的潜在替代移植物。
