Liu Xinhong, Fan Jian-Bo, Hu Jing, Li Fang, Yi Ruokun, Tan Fang, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing China.
Chongqing Engineering Research Center of Functional Food Chongqing University of Education Chongqing China.
Food Sci Nutr. 2020 Aug 13;8(9):5182-5191. doi: 10.1002/fsn3.1824. eCollection 2020 Sep.
Using retinoic acid to inducer, we successfully established a rat model of secondary osteoporosis and verified the preventive effect of ZS40 (ZS40) on secondary osteoporosis. Serum biochemical indicators showed that ZS40 can effectively slow down bone resorption caused by retinoic acid, increase blood content of calcium, phosphorus, bone alkaline phosphatase, bone gla protein, and insulin-like growth factor 1, and decrease blood content of tartrate-resistant acid phosphatase (TRAP) 5b. qRT-PCR results showed that ZS40 could upregulate mRNA expressions of , , , , , , , and , and downregulate mRNA expression of , , and in the rats' spinal cord. Results following TRAP staining showed that ZS40 could slow down retinoic acid-induced formation of osteoclasts. Micro-CT results showed that ZS40 could reduce Tb.Sp, increase BV/TV, Tb.N, Tb.Th, and ultimately increase bone mineral density of rats in vivo. These findings indicate that ZS40 might have a potential role in preventing retinoic acid-induced secondary osteoporosis in vivo.
以维甲酸为诱导剂，我们成功建立了大鼠继发性骨质疏松模型，并验证了ZS40对继发性骨质疏松的预防作用。血清生化指标显示，ZS40可有效减缓维甲酸引起的骨吸收，增加血钙、磷、骨碱性磷酸酶、骨钙素和胰岛素样生长因子1的血含量，并降低抗酒石酸酸性磷酸酶（TRAP）5b的血含量。qRT-PCR结果显示，ZS40可上调大鼠脊髓中 、 、 、 、 、 、 和 的mRNA表达，并下调 、 和 的mRNA表达。TRAP染色结果显示，ZS40可减缓维甲酸诱导的破骨细胞形成。显微CT结果显示，ZS40可降低骨小梁间距（Tb.Sp），增加骨体积分数（BV/TV）、骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th），最终增加大鼠体内骨密度。这些发现表明，ZS40在体内预防维甲酸诱导的继发性骨质疏松方面可能具有潜在作用。
