Lee Ki-Sun, Jeon Yu-Sung, Shin Sang-Wan, Lee Jeong-Yol
Department of Prosthodontics, Korea University Guro Hospital, Seoul, Republic of Korea.
Graduate School of Clinical Dentistry, Korea University, Seoul, Republic of Korea.
Biomed Res Int. 2017;2017:7141296. doi: 10.1155/2017/7141296. Epub 2017 Oct 17.
The aim of this study was to evaluate the efficacy of growth factor loaded collagen membranes on new bone formation during horizontal bone augmentation. Mandibular defects (4 × 4 × 4 mm) were surgically prepared in six male beagle dogs, which were then protected with one of three types of membranes: (1) titanium mesh, (2) titanium reinforced collagen, or (3) rhBMP-2 loaded titanium reinforced collagen. Animals were euthanized 8 and 16 weeks after surgery, and nondecalcified specimens were prepared and histomorphologically investigated to determine the degree of osteogenesis. Data were analyzed with Friedman test. With respect to the degree of osteogenesis at earlier stage (8 weeks after surgery), there was significantly higher new bone ratio in rhBMP-2 loaded membrane group ( > 0.05). However, with respect to the long-term results (16 weeks after surgery), there were no significant differences among the three membranes ( > 0.05). Based on histomorphometric analysis, there were no significant differences in horizontal bone gaining ratio ( > 0.05).
本研究的目的是评估负载生长因子的胶原膜在水平骨增量过程中对新骨形成的疗效。在六只雄性比格犬身上通过手术制造下颌骨缺损（4×4×4毫米），然后用三种类型的膜之一进行覆盖：（1）钛网，（2）钛增强胶原，或（3）负载重组人骨形态发生蛋白-2（rhBMP-2）的钛增强胶原。在术后8周和16周对动物实施安乐死，制备未脱钙标本并进行组织形态学研究以确定成骨程度。数据采用弗里德曼检验进行分析。关于早期阶段（术后8周）的成骨程度，负载rhBMP-2的膜组新骨比例显著更高（>0.05）。然而，关于长期结果（术后16周），三种膜之间没有显著差异（>0.05）。基于组织形态计量学分析，水平骨增益率没有显著差异（>0.05）。
