Coeliac Disease Research Center, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland.
Acta Derm Venereol. 2018 Mar 13;98(3):366-372. doi: 10.2340/00015555-2849.
Coeliac disease and dermatitis herpetiformis (DH) are characterized by autoantibodies targeting transglutaminase (TG)2 and TG3, respectively. Previous studies show that TG2 antibodies are produced in the gut and can be assessed in organ culture of small-intestinal biopsies from patients with coeliac disease. Thus far, no studies have investigated TG3 antibodies in organ culture of biopsies from patients with DH, or exploited the method in DH. The aim of this study was to investigate TG3 and TG2 antibody responses in serum and small-intestinal biopsies from patients with DH with active disease, and from those in remission. The majority of patients with DH were negative for both serum and organ culture medium TG2-targeting antibodies. Surprisingly, patients with active DH secreted TG3 antibodies into the culture medium despite seronegativity. In patients secreting high levels of TG3 antibodies into the culture medium, we also detected TG3-antibody-positive cells in the small-intestinal mucosa. These findings suggest that TG3 antibodies can be investigated in the organ culture system and that their secretion occurs in the small intestine, especially in active DH.
乳糜泻和疱疹样皮炎（DH）的特征分别是针对转谷氨酰胺酶（TG）2 和 TG3 的自身抗体。先前的研究表明，TG2 抗体在肠道中产生，可以在乳糜泻患者的小肠活检的器官培养物中进行评估。迄今为止，尚无研究调查过 DH 患者活检的器官培养物中的 TG3 抗体，或在 DH 中利用该方法。本研究旨在调查活动性 DH 患者和缓解期 DH 患者的血清和小肠活检中 TG3 和 TG2 抗体的反应。大多数 DH 患者的血清和器官培养物中的 TG2 靶向抗体均为阴性。令人惊讶的是，尽管血清阴性，但活动性 DH 患者会将 TG3 抗体分泌到培养基中。在向培养基中分泌高水平 TG3 抗体的患者中，我们还在小肠黏膜中检测到 TG3 抗体阳性细胞。这些发现表明，可以在器官培养系统中研究 TG3 抗体，并且其分泌发生在小肠中，尤其是在活动性 DH 中。
