Department of Chemistry and Biomolecular Science, Clarkson University , Potsdam, New York 13699-5810, United States.
Institute for Molecular Bioscience, The University of Queensland , Brisbane QLD 4072, Australia.
J Phys Chem B. 2017 Dec 28;121(51):11465-11471. doi: 10.1021/acs.jpcb.7b11151. Epub 2017 Dec 14.
An artificial Ca-regulated PQQ glucose dehydrogenase (PQQ-GDH) enzyme was electrically connected to conducting electrodes and semiconductor interfaces. Direct electron transfer from the enzyme to the conducting electrode support was stimulated by the addition of Ca cations resulting in reversible enzyme activation. A signal-switchable biofuel cell and biomolecular release have been realized using the Ca-activated enzyme immobilized on conducting electrodes. Interfacing the signal-switchable enzyme with a semiconductor chip allowed electronic read out of the enzyme ON-OFF states. The developed approach based on the signal-regulated PQQ-GDH enables numerous bioelectrochemical/bioelectronic applications of the developed systems in signal-activated biosensors and biofuel cells, as well as in biomolecular computing/logic systems.
人工 Ca 调节的 PQQ 葡萄糖脱氢酶 (PQQ-GDH) 酶与导电电极和半导体界面电连接。通过添加 Ca 阳离子刺激酶向导电电极支持物的直接电子转移，从而实现酶的可逆激活。使用固定在导电电极上的 Ca 激活酶实现了信号可切换的生物燃料电池和生物分子释放。将信号可切换酶与半导体芯片接口连接，允许对酶的 ON-OFF 状态进行电子读取。基于信号调节的 PQQ-GDH 的开发方法使得在信号激活生物传感器和生物燃料电池以及生物分子计算/逻辑系统中，对开发系统进行许多生物电化学/生物电子应用成为可能。
