Gyulev Ivan S, Willson Benjamin J, Hennessy Rosanna C, Krabben Preben, Jenkinson Elizabeth R, Thomas Gavin H
Department of Biology, University of York , Wentworth Way, York YO10 5DD, United Kingdom.
Department of Plant and Environmental Sciences, University of Copenhagen , Frederiksberg C, 1871, Denmark.
ACS Synth Biol. 2018 Feb 16;7(2):311-327. doi: 10.1021/acssynbio.7b00327. Epub 2017 Dec 11.
The solventogenic Clostridia are of interest to the chemical industry because of their natural ability to produce chemicals such as butanol, acetone and ethanol from diverse feedstocks. Their use as whole cell factories presents multiple metabolic engineering targets that could lead to improved sustainability and profitability of Clostridium industrial processes. However, engineering efforts have been held back by the scarcity of genetic and synthetic biology tools. Over the past decade, genetic tools to enable transformation and chromosomal modifications have been developed, but the lack of a broad palette of synthetic biology parts remains one of the last obstacles to the rapid engineered improvement of these species for bioproduction. We have systematically reviewed existing parts that have been used in the modification of solventogenic Clostridia, revealing a narrow range of empirically chosen and nonengineered parts that are in current use. The analysis uncovers elements, such as promoters, transcriptional terminators and ribosome binding sites where increased fundamental knowledge is needed for their reliable use in different applications. Together, the review provides the most comprehensive list of parts used and also presents areas where an improved toolbox is needed for full exploitation of these industrially important bacteria.
产溶剂梭菌对化学工业具有重要意义，因为它们具有从多种原料中天然生产丁醇、丙酮和乙醇等化学品的能力。将其用作全细胞工厂提出了多个代谢工程目标，这些目标可能会提高梭菌工业生产过程的可持续性和盈利能力。然而，由于遗传和合成生物学工具的匮乏，工程方面的努力受到了阻碍。在过去十年中，已经开发出了用于转化和染色体修饰的遗传工具，但缺乏广泛的合成生物学元件仍然是这些物种用于生物生产的快速工程改良的最后障碍之一。我们系统地回顾了用于修饰产溶剂梭菌的现有元件，发现目前使用的元件范围狭窄，是根据经验选择的且未经工程设计。该分析揭示了一些元件，如启动子、转录终止子和核糖体结合位点，在不同应用中可靠使用这些元件需要更多的基础知识。总之，该综述提供了最全面的已使用元件列表，同时也指出了为充分利用这些具有工业重要性的细菌而需要改进工具箱的领域。
