Green Biologics Ltd, R&D labs, 154AH Brook Drive, Milton Park, Abingdon OX14 4SD, UK.
School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.
FEMS Microbiol Lett. 2019 Mar 1;366(6). doi: 10.1093/femsle/fnz059.
The solventogenic clostridia have long been known for their ability to convert sugars from complex feedstocks into commercially important solvents. Although the acetone-butanol-ethanol process fell out of favour decades ago, renewed interest in sustainability and 'green' chemistry has re-established our appetite for reviving technologies such as these, albeit with 21st century improvements. As CRISPR-Cas genome editing tools are being developed and applied to the solventogenic clostridia, their industrial potential is growing. Through integration of new pathways, the beneficial traits and historical track record of clostridial fermentation can be exploited to generate a much wider range of industrially relevant products. Here we show the application of genome editing using the endogenous CRISPR-Cas mechanism of Clostridium saccharoperbutylacetonicum N1-4(HMT), to generate a deletion, SNP and to integrate new DNA into the genome. These technological advancements pave the way for application of clostridial species to the production of an array of products.
产溶剂梭菌长期以来以其将复杂原料中的糖转化为具有商业重要性的溶剂的能力而闻名。尽管丙酮丁醇乙醇工艺在几十年前就已经失宠，但对可持续性和“绿色”化学的重新关注重新激发了我们对这些技术的兴趣，尽管是在 21 世纪进行了改进。随着 CRISPR-Cas 基因组编辑工具的开发和应用于产溶剂梭菌，其工业潜力正在增长。通过整合新途径，可以利用梭菌发酵的有益特性和历史记录来生成更广泛的工业相关产品。在这里，我们展示了使用 Clostridium saccharoperbutylacetonicum N1-4(HMT) 的内源性 CRISPR-Cas 机制进行基因组编辑的应用，以产生缺失、SNP 并将新 DNA 整合到基因组中。这些技术进步为应用梭菌生产一系列产品铺平了道路。
