Bertrand D, Bader C R, Distasi C, Forster I C
Département de Physiologie, CMU, Genève, Switzerland.
J Neurosci Methods. 1989 Jan;26(3):233-8. doi: 10.1016/0165-0270(89)90121-0.
A device which can generate rectangular currents in the picoampere range is described. The current generator is a photodiode connected to the head stage of a single-channel recording amplifier. The photodiode is activated by a light-emitting diode controlled by a computer or any other current source. The device can transmit signals corresponding to simulated single-channel behaviour. Since the kinetic parameters of the simulation are known, the user can test the data acquisition and analysis system under conditions similar to those prevailing during recording from a biological membrane. This current generator can also be used for the tuning of patch-clamp amplifiers; rectangular currents generated by the photodiode allow the frequency response of the amplifier to be properly adjusted.
本文描述了一种能够产生皮安级矩形电流的装置。该电流发生器是一个连接到单通道记录放大器前置级的光电二极管。光电二极管由计算机或任何其他电流源控制的发光二极管激活。该装置可以传输与模拟单通道行为相对应的信号。由于模拟的动力学参数是已知的，用户可以在类似于从生物膜记录时的条件下测试数据采集和分析系统。这种电流发生器还可用于膜片钳放大器的调谐；光电二极管产生的矩形电流可使放大器的频率响应得到适当调整。
