一种混合膜片钳放大器。

A hybrid patch clamp amplifier.

作者信息

Strickholm A

机构信息

Medical Sciences Program, Indiana University, Bloomington 47405.

出版信息

J Neurosci Methods. 1989 Jul;29(1):59-67. doi: 10.1016/0165-0270(89)90108-8.

DOI:10.1016/0165-0270(89)90108-8

PMID:2761298

Abstract

The current-to-voltage convertor used in patch clamping is analyzed for noise generation and the major noise sources determined. A hybrid patch clamp amplifier design is theoretically analyzed. Here it is shown that by differentiation and recombining of signals the original input signal can be reconstructed. Several circuits of this design are described and their performance compared. The optimal signal detection obtained for a 1 ms current pulse width with a signal-to-noise ratio of 1 is 0.025 pA. In these circuits, high frequency attenuation is readily accomplished with a single control. In addition, compensation for the transients which occur with step control voltages is effectively accomplished. With this circuitry, it is shown that for most patch clamp situations, the minimum pulse width and current which can be detected is determined by the patch clamp seal resistance.

摘要

对膜片钳技术中使用的电流-电压转换器进行了噪声产生分析，并确定了主要噪声源。从理论上分析了一种混合膜片钳放大器设计。结果表明，通过信号的微分和重组可以重构原始输入信号。描述了该设计的几种电路，并比较了它们的性能。对于1 ms的电流脉冲宽度，信噪比为1时获得的最佳信号检测值为0.025 pA。在这些电路中，通过单一控制即可轻松实现高频衰减。此外，还能有效补偿阶跃控制电压时出现的瞬态。利用该电路表明，对于大多数膜片钳情况，可检测的最小脉冲宽度和电流由膜片钳封接电阻决定。