• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

用于控制膜片钳实验的带遥控器的多功能补充装置。

Versatile supplement device with remote control for the control of patch clamp experiments.

作者信息

Rohlicek V, Fröbe U, Gögelein H, Greger R

机构信息

Physiological Institute CSAV, Prague, Czechoslovakia.

出版信息

Pflugers Arch. 1989 Feb;413(4):444-6. doi: 10.1007/BF00584498.

DOI:10.1007/BF00584498
PMID:2928099
Abstract

A versatile device for a patch-clamp amplifier is described. This device contains: (i) an acoustic indicator to monitor the input resistance of the patch pipette, which is used in search-mode to indicate the formation of seals; (ii) two pulse generators; and (iii) a staircase generator to produce various pulse and voltage step programs; (iv) a low-pass filter which is used to filter the output of the patch clamp amplifier; and (v) a remote control which is used to control the entire patch clamp experiment. This remote control is used to switch between search-, current clamp-, and voltage clamp-mode, to activate the respective stimulus potential programs, and to control the tape recorder. This electronic device can be easily connected to patch clamp amplifiers.

摘要

描述了一种用于膜片钳放大器的多功能设备。该设备包括:(i) 一个声学指示器,用于监测膜片移液管的输入电阻,在搜索模式下用于指示封接的形成;(ii) 两个脉冲发生器;(iii) 一个阶梯发生器,用于产生各种脉冲和电压阶跃程序;(iv) 一个低通滤波器,用于过滤膜片钳放大器的输出;以及(v) 一个遥控器,用于控制整个膜片钳实验。该遥控器用于在搜索模式、电流钳模式和电压钳模式之间切换,激活相应的刺激电位程序,并控制磁带录音机。这种电子设备可以很容易地连接到膜片钳放大器上。

相似文献

1
Versatile supplement device with remote control for the control of patch clamp experiments.用于控制膜片钳实验的带遥控器的多功能补充装置。
Pflugers Arch. 1989 Feb;413(4):444-6. doi: 10.1007/BF00584498.
2
A hybrid patch clamp amplifier.一种混合膜片钳放大器。
J Neurosci Methods. 1989 Jul;29(1):59-67. doi: 10.1016/0165-0270(89)90108-8.
3
Construction, calibration, and validation of a simple patch-clamp amplifier for physiology education.构建、校准和验证用于生理学教育的简单膜片钳放大器。
Adv Physiol Educ. 2009 Jun;33(2):121-9. doi: 10.1152/advan.90205.2008.
4
An inexpensive, high output voltage, voltage clamp for epithelial membranes.
Am J Physiol. 1978 Jul;235(1):C69-72. doi: 10.1152/ajpcell.1978.235.1.C69.
5
Patch voltage clamping with low-resistance seals: loose patch clamp.
Methods Enzymol. 1992;207:155-76. doi: 10.1016/0076-6879(92)07011-c.
6
Single-channel current simulation and recording using a photodiode as current generator.使用光电二极管作为电流发生器进行单通道电流模拟与记录。
J Neurosci Methods. 1989 Jan;26(3):233-8. doi: 10.1016/0165-0270(89)90121-0.
7
Enhanced Monitoring of Nanosecond Electric Pulse-Evoked Membrane Conductance Changes in Whole-Cell Patch Clamp Experiments.全细胞膜片钳实验中对纳秒级电脉冲诱发的膜电导变化的增强监测。
J Membr Biol. 2016 Oct;249(5):633-644. doi: 10.1007/s00232-016-9902-5. Epub 2016 Apr 13.
8
Operational amplifier with adjustable frequency response.频率响应可调的运算放大器。
Physiol Bohemoslov. 1978;27(2):179-84.
9
A pipette holder for use in patch-clamp measurements.
J Neurosci Methods. 1990 Feb;31(2):89-91. doi: 10.1016/0165-0270(90)90152-6.
10
A low-cost voltage stepper device for use in patch-clamp experiments.一种用于膜片钳实验的低成本电压步进装置。
Pflugers Arch. 1987 Feb;408(2):194-5. doi: 10.1007/BF00581351.

引用本文的文献

1
Effect of NH4+/NH3 on cytosolic pH and the K+ channels of freshly isolated cells from the thick ascending limb of Henle's loop.NH4+/NH3对来自髓袢升支粗段新鲜分离细胞的胞质pH及钾通道的影响。
Pflugers Arch. 1995 Jan;429(3):345-54. doi: 10.1007/BF00374149.
2
Effects of cell differentiation on ion conductances and membrane voltage in LLC-PK1 cells.细胞分化对LLC-PK1细胞离子电导和膜电压的影响。
Pflugers Arch. 1995 Jan;429(3):370-7. doi: 10.1007/BF00374152.
3
Properties and regulation of chloride channels in cystic fibrosis and normal airway cells.

本文引用的文献

1
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.用于从细胞和无细胞膜片进行高分辨率电流记录的改进膜片钳技术。
Pflugers Arch. 1981 Aug;391(2):85-100. doi: 10.1007/BF00656997.
2
A low-cost voltage stepper device for use in patch-clamp experiments.一种用于膜片钳实验的低成本电压步进装置。
Pflugers Arch. 1987 Feb;408(2):194-5. doi: 10.1007/BF00581351.
3
Na+ selective channels in the apical membrane of rabbit late proximal tubules (pars recta).
囊性纤维化和正常气道细胞中氯离子通道的特性与调控
Pflugers Arch. 1989 Nov;415(2):172-82. doi: 10.1007/BF00370589.
4
The luminal K+ channel of the thick ascending limb of Henle's loop.
Pflugers Arch. 1990 Jan;415(4):449-60. doi: 10.1007/BF00373623.
5
Effect of depolarizing and hyperpolarizing agents on the membrane potential difference of primary cultures of rabbit aorta vascular smooth muscle cells.去极化剂和超极化剂对兔主动脉血管平滑肌细胞原代培养物膜电位差的影响。
Pflugers Arch. 1991 Aug;419(1):69-75. doi: 10.1007/BF00373749.
6
Potassium conductance of smooth muscle cells from rabbit aorta in primary culture.原代培养的兔主动脉平滑肌细胞的钾离子电导
Pflugers Arch. 1991 Aug;419(1):57-68. doi: 10.1007/BF00373748.
7
Bicarbonate permeability of epithelial chloride channels.上皮氯离子通道的碳酸氢根通透性
Pflugers Arch. 1991 Feb;417(6):616-21. doi: 10.1007/BF00372960.
8
Different types of blockers of the intermediate-conductance outwardly rectifying chloride channel in epithelia.上皮细胞中不同类型的中电导外向整流氯离子通道阻滞剂。
Pflugers Arch. 1991 Jul;418(6):556-63. doi: 10.1007/BF00370571.
9
Sodium-alanine cotransport in renal proximal tubule cells investigated by whole-cell current recording.通过全细胞电流记录研究肾近端小管细胞中的钠-丙氨酸共转运。
J Gen Physiol. 1991 May;97(5):1073-94. doi: 10.1085/jgp.97.5.1073.
10
Activation of nonselective cation channels in the basolateral membrane of rat distal colon crypt cells by prostaglandin E2.
Pflugers Arch. 1992 Mar;420(3-4):319-28. doi: 10.1007/BF00374465.
Pflugers Arch. 1986 Feb;406(2):198-203. doi: 10.1007/BF00586683.
4
Single-channel currents recorded from membrane of denervated frog muscle fibres.从去神经支配的蛙肌纤维膜记录的单通道电流。
Nature. 1976 Apr 29;260(5554):799-802. doi: 10.1038/260799a0.