Bingel M, Arndt W, Schulze M, Floege J, Shaldon S, Koch K M, Götze O
Department of Nephrology, Hannover Medical School, FRG.
Nephron. 1989;51(3):320-4. doi: 10.1159/000185316.
Using a specific and sensitive ELISA for C5a, the present study shows that predialysis levels of C5a in end-stage renal disease patients are not elevated and that all membranes studied (Cuprophan, Hemophan, Gambrane and hydrophilic polysulfone) cause significant increases of C5a plasma levels albeit to different degrees. Higher increases of C5a were accompanied by larger decreases of circulating granulocytes and monocytes. Dialyzers with a newly introduced modified regenerated cellulose membrane, Hemophan, showed lower C5a plasma levels during hemodialysis than Cuprophan. With Hemophan C5a plasma levels were comparable to those with polysulfone membranes.
通过使用一种针对C5a的特异性且灵敏的酶联免疫吸附测定法（ELISA），本研究表明，终末期肾病患者透析前的C5a水平并未升高，并且所有研究的膜（铜仿膜、血仿膜、伽马膜和亲水性聚砜膜）都会导致C5a血浆水平显著升高，尽管程度不同。C5a升高幅度越大，循环粒细胞和单核细胞减少幅度就越大。新推出的改良再生纤维素膜血仿膜制成的透析器在血液透析过程中显示出的C5a血浆水平低于铜仿膜。使用血仿膜时C5a血浆水平与使用聚砜膜时相当。
