人房水的鸟枪法鞘脂分析
Shotgun Sphingolipid Analysis of Human Aqueous Humor.
作者信息
Trzeciecka Anna, Arbelo Ulises, Barron Arturo, Edwards Genea, Sampathkumar Sruthi, Toris Carol, Bhattacharya Sanjoy K
机构信息
Bascom Palmer Eye Institute, McKnight Vision Research Center, University of Miami, Miami, FL, USA.
Bascom Palmer Eye Institute, University of Miami, Miami, FL, USA.
出版信息
Methods Mol Biol. 2018;1695:97-107. doi: 10.1007/978-1-4939-7407-8_10.
Abstract
This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.
摘要
本方案提供了一份用于房水鞘脂分析的逐步指南。我们描述了布利格和戴尔粗脂质提取方法以及电喷雾电离串联质谱（ESI-MS/MS），并结合MZmine 2.21数据处理，用于鞘氨醇、鞘氨醇-1-磷酸、鞘磷脂和神经酰胺的鉴定和比例定量。
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