Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine , Miami, Florida.
J Ocul Pharmacol Ther. 2014 Mar-Apr;30(2-3):283-90. doi: 10.1089/jop.2013.0168. Epub 2013 Dec 9.
To determine the differential profiles of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramide and their quantitative differences between trabecular meshwork (TM) derived from normotensive and hypertensive intraocular pressure states of DBA/2J mice.
Normotensive and hypertensive state TM were collected from mice and analyzed. Lipid extraction was performed using the Bligh and Dyer method, and the protein concentrations were determined using the Bradford method. The lipids were identified and quantified using appropriate standards with a TSQ Quantum Access Max triple quadrupole mass spectrometer applying class-specific lipid identification settings.
The comparative profiles of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramide between normotensive and hypertensive TM showed several species unique to a phase and as well common between states.
The presence or absence of several sphingolipids and ceramides in the normotensive or hypertensive states may contribute to better understanding of the glaucomas.
确定来自正常眼压和高血压眼压状态下 DBA/2J 小鼠小梁网的神经酰胺及其定量差异的神经鞘磷脂、神经鞘氨醇碱基、神经鞘氨醇碱基-1-磷酸和神经酰胺的差异特征。
从小鼠中收集正常眼压和高血压状态下的 TM 并进行分析。使用 Bligh 和 Dyer 法进行脂质提取，并使用 Bradford 法测定蛋白质浓度。使用适当的标准品，应用具有特定脂质鉴定设置的 TSQ Quantum Access Max 三重四极杆质谱仪对脂质进行鉴定和定量。
正常眼压和高血压 TM 之间神经鞘磷脂、神经鞘氨醇碱基、神经鞘氨醇碱基-1-磷酸和神经酰胺的比较图谱显示了一些在相位上特有的物种，以及在状态之间共同存在的物种。
正常或高血压状态下某些神经鞘脂和神经酰胺的存在或缺失可能有助于更好地理解青光眼。
