Ishibashi T, Sugai S, Kubota T, Ohnishi Y, Inomata H
Department of Ophthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ophthalmologica. 1989;198(1):30-4. doi: 10.1159/000309955.
We developed a new method for specimen preparation for transmission electronmicroscopic study of cellular responses on intraocular lenses. This procedure omits propylene oxide treatment of the materials to be studied, which is normally a step in the routine preparation process for transmission electron microscopy. It was previously found that propylene oxide treatment dissolves intraocular lenses. Using our procedure, two types of cells were identified on intraocular lenses removed from monkey eyes 7 days after implantation. These cells had the typical structural features of multinucleated giant cells and of macrophages. It thus appears that this procedure will permit study of the cytology of intraocular lens surfaces.
我们开发了一种新的标本制备方法，用于对人工晶状体的细胞反应进行透射电子显微镜研究。该程序省略了对待研究材料的环氧丙烷处理，而环氧丙烷处理通常是透射电子显微镜常规制备过程中的一个步骤。先前发现环氧丙烷处理会溶解人工晶状体。使用我们的程序，在植入后7天从猴眼中取出的人工晶状体上鉴定出了两种类型的细胞。这些细胞具有多核巨细胞和巨噬细胞的典型结构特征。因此，看来该程序将允许对人工晶状体表面的细胞学进行研究。
