Uenoyama K, Kanagawa R, Tamura M, Matoba M, Enomoto Y, Ohmi S
Department of Ophthalmology, Wakayama Medical College, Japan.
J Cataract Refract Surg. 1989 Sep;15(5):559-66. doi: 10.1016/s0886-3350(89)80116-6.
Transmission and scanning electron microscopy, Wolter's implant cytology staining, and an immunohistochemical method were used to investigate the process of cell adhesion, the origin of fibroblast-like cells, and the nature of lymphocytic clusters that were observed on intraocular lenses (IOLs) experimentally implanted in the rabbit eye and in the mouse peritoneal space. On the IOL implanted in the mouse peritoneal space, pseudopodia extended during cell adhesion showed morphological variety; on the IOL implanted in the rabbit eye, membranous extensions were seen. Many of the fibroblast-like cells exhibited positive staining for macrophagic antigen, indicating a macrophagic origin. The build-up of lymphocytic clusters, as an indicator of immunologic activity, was frequently observed on IOLs implanted in the mouse peritoneal space, particularly on silicone IOLs. However, such clusters were rarely seen on poly(methyl methacrylate) or silicone IOLs implanted in the rabbit eye, suggesting a much reduced immune response to those materials in the eye chamber.
采用透射电子显微镜和扫描电子显微镜、沃尔特植入物细胞学染色以及免疫组织化学方法，研究了细胞黏附过程、成纤维细胞样细胞的起源以及在实验性植入兔眼和小鼠腹腔的人工晶状体（IOL）上观察到的淋巴细胞簇的性质。在植入小鼠腹腔的人工晶状体上，细胞黏附过程中伸出的伪足形态多样；在植入兔眼的人工晶状体上，则可见膜状延伸。许多成纤维细胞样细胞对巨噬细胞抗原呈阳性染色，表明其起源于巨噬细胞。作为免疫活性指标的淋巴细胞簇的形成，在植入小鼠腹腔的人工晶状体上经常观察到，尤其是在硅酮人工晶状体上。然而，在植入兔眼的聚甲基丙烯酸甲酯或硅酮人工晶状体上很少见到此类簇，这表明眼房对这些材料的免疫反应大大降低。
