Alemán-Ávila Isidro, Jiménez-Morales Mayra, Beltrán-Ramírez Olga, Barbosa-Cobos Rosa Elda, Jiménez-Morales Silvia, Sánchez-Muñoz Fausto, Valencia-Pacheco Guillermo, Amezcua-Guerra Luis M, Juárez-Vicuña Yaneli, Razo-Blanco Hernández Dulce Milagro, Aguilera-Cartas María Concepción, López-Villanueva Ricardo F, Peralta-Zaragoza Oscar, Tovilla-Zárate Carlos, Ramírez-Bello Julian
Endocrine and Metabolic Disease Unit Research, Hospital Juarez of Mexico, Mexico City, Mexico.
Superior School of Medicine Postgraduate Program, National Polytechnic Institute, Mexico City, Mexico.
Oncotarget. 2017 Jul 27;8(54):91876-91886. doi: 10.18632/oncotarget.19621. eCollection 2017 Nov 3.
Recently, different microRNA (miRNA) gene polymorphisms have been evaluated in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and Graves' disease (GD). In the present study, we examined three single-nucleotide polymorphisms (SNPs) located in the (rs2910164G/C), (rs11614913C/T), and (rs3746444A/G) genes. Our study population included 900 Mexican patients with RA, SLE, or GD, as well as 486 healthy control individuals with no family history of inflammatory or autoimmune diseases. Genotyping was performed using TaqMan probes and a 5' exonuclease assay. None of the investigated SNPs were associated with RA or GD susceptibility under any genetic model (co-dominant, recessive, or dominant). Genotype and allele frequencies of the rs11614913C/T polymorphism were similar between SLE cases and controls. In contrast, the rs2910164G/C and rs3746444A/G polymorphisms were associated with SLE susceptibility. These SNPs were not associated with lupus nephritis (LN). Our results suggest that polymorphisms in , and are not associated with RA or GD susceptibility. This is the first report documenting that the rs2910164G/C and rs3746444 polymorphisms are associated with SLE susceptibility but not with LN.
最近，研究人员对类风湿性关节炎（RA）、系统性红斑狼疮（SLE）和格雷夫斯病（GD）患者的不同微小RNA（miRNA）基因多态性进行了评估。在本研究中，我们检测了位于 （rs2910164G/C）、 （rs11614913C/T）和 （rs3746444A/G）基因中的三个单核苷酸多态性（SNP）。我们的研究对象包括900名患有RA、SLE或GD的墨西哥患者，以及486名无炎症或自身免疫性疾病家族史的健康对照个体。使用TaqMan探针和5'核酸外切酶测定法进行基因分型。在任何遗传模型（共显性、隐性或显性）下，所研究的SNP均与RA或GD易感性无关。SLE病例和对照之间rs11614913C/T多态性的基因型和等位基因频率相似。相比之下，rs2910164G/C和rs3746444A/G多态性与SLE易感性相关。这些SNP与狼疮性肾炎（LN）无关。我们的结果表明， 、 和 中的多态性与RA或GD易感性无关。这是第一份记录rs2910164G/C和rs3746444多态性与SLE易感性相关但与LN无关的报告。
