Department of Immunology, Faculty of Medicine, Medical University of Gdańsk, Dębinki 1, 80-210, Gdańsk, Poland.
University Center for Cardiology Department of Family Medicine, Medical University of Gdansk, M. Skłodowskiej-Curie 3a, 80-210, Gdańsk, Poland.
J Hum Hypertens. 2017 Dec;32(1):46-53. doi: 10.1038/s41371-017-0020-3. Epub 2017 Nov 30.
Populations of CD34- and VEGFR2-expressing cells are responsible for regeneration of damaged endothelium and vascular remodelling. As their quantity and activity changes during cardiovascular diseases, they are potentially useful markers of cardiovascular health. The aim of our study was to investigate changes of various CD34+ and CD34+ VEGFR2+ populations in subjects with newly recognised hypertension and to evaluate whether observed alterations are influenced by clinical parameters and angiotensin II. Circulating CD34+ and CD34+ VEGFR2+ cells were analysed in peripheral blood samples by flow cytometry. Serum levels of angiotensin II were determined using immunoenzymatic assay. We discovered increased proportions of various CD34+ populations and CD34+ VEGFR2+ c-Kit+ cells in newly diagnosed patients. CD34+ cells seem to be influenced by angiotensin II, but we did not observe comparable results when populations co-expressing VEGFR2 were analysed. The quantity of CD34+ VEGFR2+ cells in patients with newly recognised primary hypertension ought to be determined by other factors. Increased proportions of CD34+ progenitors in blood could comprise compensatory mechanism for increased endothelial damage in hypertension.
CD34- 和 VEGFR2- 表达细胞群体负责受损内皮的再生和血管重塑。由于它们的数量和活性在心血管疾病期间发生变化，因此它们是心血管健康的潜在有用标志物。我们的研究目的是研究新诊断的高血压患者中各种 CD34+ 和 CD34+ VEGFR2+ 群体的变化，并评估观察到的改变是否受临床参数和血管紧张素 II 的影响。通过流式细胞术分析外周血样本中的循环 CD34+ 和 CD34+ VEGFR2+ 细胞。使用免疫酶测定法测定血管紧张素 II 的血清水平。我们发现新诊断的患者中各种 CD34+ 群体和 CD34+ VEGFR2+ c-Kit+ 细胞的比例增加。CD34+ 细胞似乎受血管紧张素 II 的影响，但当分析同时表达 VEGFR2 的群体时，我们没有观察到可比的结果。新诊断原发性高血压患者中 CD34+ VEGFR2+ 细胞的数量可能由其他因素决定。血液中 CD34+ 祖细胞的比例增加可能构成高血压中内皮损伤增加的代偿机制。
