一种使用动态锰增强磁共振成像评估小鼠肾脏活力的快速 T 映射方法。
A rapid T mapping method for assessment of murine kidney viability using dynamic manganese-enhanced magnetic resonance imaging.
机构信息
Division of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, USA.
Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA.
出版信息
Magn Reson Med. 2018 Jul;80(1):190-199. doi: 10.1002/mrm.27025. Epub 2017 Nov 28.
PURPOSE
Dynamic manganese-enhanced MRI (MEMRI) allows assessment of tissue viability by tracing manganese uptake. We aimed to develop a rapid T mapping method for dynamic MEMRI to facilitate assessments of murine kidney viability.
METHODS
A multi-slice saturation recovery fast spin echo (MSRFSE) was developed, validated, and subsequently applied in dynamic MEMRI at 16.4T on ischemic mouse kidneys after 4 weeks of unilateral renal artery stenosis (RAS). Baseline T values and post-contrast R (1/T ) changes were measured in cortex (CO), outer (OSOM), inner (ISOM) strips of outer medulla, and inner medulla (IM).
RESULTS
Validation studies showed strong agreement between MSRFSE and an established saturation recovery Look-Locker method. Baseline T (s) increased in the stenotic kidney CO (2.10 [1.95-2.56] vs. 1.88 [1.81-2.00], P = 0.0317) and OSOM (2.17 [2.05-2.33] vs. 1.96 [1.87-2.00], P = 0.0075) but remained unchanged in ISOM and IM. This method allowed a temporal resolution of 1.43 min in dynamic MEMRI. Mn uptake and retention decreased in stenotic kidneys, particularly in the OSOM (ΔR : 0.48 [0.38-0.56] vs. 0.64 [0.61-0.69] s , P < 0.0001).
CONCLUSION
Dynamic MEMRI by MSRFSE detected decreased cellular viability and discerned the regional responses to RAS. This technique may provide a valuable tool for noninvasive evaluation of renal viability. Magn Reson Med 80:190-199, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
目的
动态锰增强 MRI（MEMRI）通过追踪锰摄取来评估组织活力。我们旨在开发一种快速 T 映射方法，用于动态 MEMRI，以方便评估小鼠肾脏的活力。
方法
开发、验证了一种多切片饱和恢复快速自旋回波（MSRFSE），并随后在 16.4T 上应用于单侧肾动脉狭窄（RAS）后 4 周的缺血性小鼠肾脏的动态 MEMRI。在皮质（CO）、外髓（OSOM）、内髓（ISOM）带和内髓（IM）中测量基线 T 值和对比后 R（1/T）变化。
结果
验证研究表明，MSRFSE 与一种成熟的饱和恢复 Look-Locker 方法具有很强的一致性。病变侧肾脏 CO（2.10[1.95-2.56]与 1.88[1.81-2.00]，P=0.0317）和 OSOM（2.17[2.05-2.33]与 1.96[1.87-2.00]，P=0.0075）的 T 值增加，但 ISOM 和 IM 无变化。该方法允许在动态 MEMRI 中达到 1.43 分钟的时间分辨率。Mn 摄取和保留减少在病变侧肾脏中，特别是在 OSOM（ΔR：0.48[0.38-0.56]与 0.64[0.61-0.69]s，P<0.0001）。
结论
通过 MSRFSE 的动态 MEMRI 检测到细胞活力降低，并分辨出对 RAS 的区域反应。该技术可能为肾脏活力的无创评估提供一种有价值的工具。磁共振医学 80：190-199，2018。©2017 年国际磁共振医学学会。
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