雨生红球藻提取物对乳腺癌细胞系的抗癌作用
The Anticancer Effect of Haematococcus Pluvialis Alga Extract on Breast Cancer Cell Line.
作者信息
Shamskhani Vahideh, Zarredar Habib, Razeghi Jafar, Ansarin Atefeh, Asadi Milad, Soleimani Zahra, Seyedrezazadeh Ensiyeh
机构信息
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Tuberculosis and Lung Diseases Research Canter, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Asian Pac J Cancer Prev. 2025 Jun 1;26(6):2071-2077. doi: 10.31557/APJCP.2025.26.6.2071.
OBJECTIVE
The aim of this study was to investigate the anticancer effects of H. pluvialis extract on breast cancer. Methods: We evaluated the cytotoxicity, suppression of migration, and apoptosis ability of H. pluvialis alga extract MDA-MB-468 by MTT assay, scratch test, and flow cytometry, respectively. The expression patterns of MMP1 and MMP9, Bax and Bcl2, Caspase3, Caspase6, and Caspase9 were analyzed by qRT-PCR.
RESULTS
Based on the findings of this study, the survival and proliferation capacity of MDA-MB-468 cells treated with the extract was reduced. Flow cytometry results showed that treatment with H. Pluvialis extracts induced apoptosis. Scratch test results showed that H. Pluvialis extract could inhibit cell migration. The expression patterns of the studied genes were changed by treatment with the extract. Expression of Bcl2, MMP1, and MMP9 genes decreased, and expression of Bax, Caspase3, Caspase8, Caspase9 genes increased.
CONCLUSION
The findings of this research reveal that H. pluvialis algae extract shows a potential anti-cancer compound.
目的
本研究旨在探讨雨生红球藻提取物对乳腺癌的抗癌作用。方法：我们分别通过MTT法、划痕试验和流式细胞术评估了雨生红球藻提取物对MDA-MB-468细胞的细胞毒性、迁移抑制和凋亡能力。通过qRT-PCR分析MMP1和MMP9、Bax和Bcl2、Caspase3、Caspase6和Caspase9的表达模式。
结果
基于本研究结果，提取物处理后的MDA-MB-468细胞的存活和增殖能力降低。流式细胞术结果表明，雨生红球藻提取物处理可诱导细胞凋亡。划痕试验结果表明，雨生红球藻提取物可抑制细胞迁移。提取物处理改变了所研究基因的表达模式。Bcl2、MMP1和MMP9基因表达降低，Bax、Caspase3、Caspase8、Caspase9基因表达增加。
结论
本研究结果表明，雨生红球藻提取物显示出一种潜在的抗癌化合物。
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