State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, Maryland, 21201.
J Biomed Mater Res B Appl Biomater. 2018 Aug;106(6):2414-2424. doi: 10.1002/jbm.b.34050. Epub 2017 Nov 28.
Patients with dry mouth often have an acidic oral environment lacking saliva to provide calcium (Ca) and phosphate (P) ions. There has been no report on tooth remineralization in acidic pH4 and CaP ion-lacking solutions. The objective of this study was to develop a novel method of combining poly(amido amine) (PAMAM) with adhesive containing nanoparticles of amorphous calcium phosphate (NACP) for dentin remineralization in pH4 and CaP-lacking solution for the first time. Demineralized dentin was tested in four groups: (1) dentin control, (2) dentin with PAMAM, (3) dentin with NACP adhesive, (4) dentin with PAMAM + NACP adhesive. Dentin samples were examined by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and hardness testing. Increasing the NACP filler level in adhesive from 0 to 40 wt% did not negatively affect the dentin bond strength (p > 0.1). NACP adhesive released CaP ions and neutralized the acid. PAMAM alone failed to achieve dentin remineralization in lactic acid. NACP alone induced slight dentin remineralization in lactic acid (p > 0.1). In contrast, the novel PAMAM + NACP group in the pH4 and CaP-lacking solution completely remineralized the predemineralized dentin, increasing its hardness which approached that of healthy dentin (p > 0.1). In conclusion, dentin remineralization via PAMAM + NACP adhesive in pH4 and CaP-lacking acid was achieved for the first time, when conventional remineralization methods such as PAMAM or NACP did not work. The novel PAMAM + NACP method is promising to increase the longevity of the composite-tooth bond, inhibit caries, remineralize lesions and protect tooth structures, even for patients with dry mouth and an acidic oral environment. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2414-2424, 2018.
口干症患者的口腔环境通常呈酸性，缺乏唾液提供钙 (Ca) 和磷酸盐 (P) 离子。目前还没有关于在酸性 pH4 和缺乏 CaP 离子的溶液中牙齿再矿化的报道。本研究的目的是首次开发一种将聚（酰胺-胺）（PAMAM）与含有纳米级无定形磷酸钙（NACP）的粘合剂结合用于在 pH4 和缺乏 CaP 的溶液中进行牙本质再矿化的新方法。脱矿牙本质在以下四组中进行测试：（1）牙本质对照组，（2）含 PAMAM 的牙本质，（3）含 NACP 粘合剂的牙本质，（4）含 PAMAM+NACP 粘合剂的牙本质。使用扫描电子显微镜 (SEM)、能谱 (EDS) 和硬度测试对牙本质样本进行了检测。将粘合剂中的 NACP 填充水平从 0 增加到 40wt% 不会对牙本质粘结强度产生负面影响（p>0.1）。NACP 粘合剂释放 CaP 离子并中和酸。单独的 PAMAM 未能在乳酸中实现牙本质再矿化。单独的 NACP 在乳酸中引起轻微的牙本质再矿化（p>0.1）。相比之下，新型 PAMAM+NACP 组在 pH4 和缺乏 CaP 的溶液中完全再矿化了预脱矿的牙本质，使其硬度增加到接近健康牙本质的水平（p>0.1）。总之，首次通过 PAMAM+NACP 粘合剂在 pH4 和缺乏 CaP 的酸性环境中实现了牙本质再矿化，而常规的再矿化方法（如 PAMAM 或 NACP）在此情况下无法发挥作用。新型 PAMAM+NACP 方法有望提高复合-牙粘结的耐久性，抑制龋齿，再矿化病变并保护牙齿结构，即使是口干症和酸性口腔环境的患者也能受益。© 2017 Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料，106B：2414-2424，2018。
