Jia Ao, Wang Pei, Tong Fei, Chen Ziqiang, Deng Yunyun, Yao Haiyan, Wang Lianguo, Liu Yifan, Ge Hongshan
The Affiliated Stomatological Hospital of Nanchang University, Nanchang 330000, China.
The Key Laboratory of Oral Biomedicine, Nanchang 330000, China.
J Funct Biomater. 2023 Jan 29;14(2):77. doi: 10.3390/jfb14020077.
During fixed orthodontic treatment, white spot lesions are prevalent issues associated with cariogenic bacteria. This study aims to construct an orthodontic adhesive containing nanoparticles of amorphous calcium phosphate-polydopamine-Ag (NPA) fillers to combat white spot lesions. The NPA fillers were prepared and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). The biocompatibility of the fillers was evaluated. A colony counting test evaluated the antibacterial property of the fillers against . NPA fillers were mixed with orthodontic adhesive (Transbond XT) at different weight ratios (0, 0.1, 0.2, 0.3, and 0.5 wt.%). The shear bond strength and antibacterial properties were then further investigated. The results showed that NPA was prepared successfully, with good antibacterial properties. The cell survival rate of all groups of fillers was higher than 70%, showing good biocompatibility. Moreover, the shear bond strength of the orthodontic adhesive with 0.2 wt.% NPA fillers was 11.89 ± 1.27 MPa, meeting the minimal clinical bond strength requirements of 7.8 MPa. Furthermore, the orthodontic adhesive resin blocks and the extract displayed good antibacterial properties, with the number of colonies decreasing significantly ( < 0.001). Taken together, we think that an orthodontic adhesive with NPA may have a good application potential for the prevention and treatment of white spot lesions.
在固定正畸治疗期间，白斑病变是与致龋菌相关的常见问题。本研究旨在构建一种含有无定形磷酸钙 - 聚多巴胺 - 银（NPA）纳米颗粒填料的正畸粘合剂，以对抗白斑病变。制备了NPA填料，并通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）对其进行了表征。评估了填料的生物相容性。通过菌落计数试验评估了填料对……的抗菌性能。将NPA填料与正畸粘合剂（Transbond XT）按不同重量比（0、0.1、0.2、0.3和0.5 wt.%）混合。然后进一步研究了剪切粘结强度和抗菌性能。结果表明，NPA制备成功，具有良好的抗菌性能。所有组填料的细胞存活率均高于70%，显示出良好的生物相容性。此外，含有0.2 wt.% NPA填料的正畸粘合剂的剪切粘结强度为11.89±1.27 MPa，满足7.8 MPa的最低临床粘结强度要求。此外，正畸粘合树脂块和提取物显示出良好的抗菌性能，菌落数量显著减少（<0.001）。综上所述，我们认为含NPA的正畸粘合剂在白斑病变的预防和治疗方面可能具有良好的应用潜力。
