赭曲霉毒素B对体外赭曲霉毒素A抑制苯丙氨酰-tRNA形成及肝癌组织培养细胞中蛋白质合成的影响。

Influence of ochratoxin B on the ochratoxin A inhibition of phenylalanyl-tRNA formation in vitro and protein synthesis in hepatoma tissue culture cells.

作者信息

Roth A, Creppy E E, Kane A, Bacha H, Steyn P S, Röschenthaler R, Dirheimer G

机构信息

Institut de Biologie Moléculaire et Cellulaire du CNRS, Université Louis Pasteur, Strasbourg, France.

出版信息

Toxicol Lett. 1989 Feb;45(2-3):307-13. doi: 10.1016/0378-4274(89)90022-2.

DOI:10.1016/0378-4274(89)90022-2

PMID:2919408

Abstract

Ochratoxin B (OTB), the dechloro-analogue of ochratoxin A (OTA), was studied separately and in combination with OTA on the aminoacylation of phenylalanine tRNA (tRNAPhe) catalysed by mice liver phenylalanyl-tRNA synthetase. OTB was neither a significant inhibitor of the reaction nor an antagonist of OTA. OTB was also assayed for its possible antagonistic effect on the in vivo protein synthesis inhibition caused by OTA in hepatoma tissue culture cells. No prevention of OTA inhibition could be found for OTB. It rather showed a slight additional inhibitory activity when mixed (100-180 microM) with low concentrations of OTA (40-60 microM). In conclusion, these results are not in favor of an antagonistic effect of OTB with respect to OTA action, at least on the level of cellular protein synthesis.

摘要

赭曲霉毒素B（OTB）是赭曲霉毒素A（OTA）的脱氯类似物，我们分别研究了它以及它与OTA联合作用对小鼠肝脏苯丙氨酰 - tRNA合成酶催化的苯丙氨酸tRNA（tRNAPhe）氨酰化反应的影响。OTB既不是该反应的显著抑制剂，也不是OTA的拮抗剂。我们还检测了OTB对OTA在肝癌组织培养细胞中引起的体内蛋白质合成抑制的可能拮抗作用。未发现OTB对OTA抑制作用有预防效果。相反，当它与低浓度（40 - 60 microM）的OTA混合（100 - 180 microM）时，反而表现出轻微的额外抑制活性。总之，这些结果不支持OTB对OTA作用具有拮抗效应，至少在细胞蛋白质合成水平上是如此。