Yoneda M, Makino I, Tamasawa N, Takebe K, Sakuraba K, Goto J, Nambara T
Third Department of Internal Medicine, Hirosaki University School of Medicine, Japan.
Am J Gastroenterol. 1989 Mar;84(3):290-5.
One gram of dehydrocholic acid was injected intravenously into two patients with percutaneous transhepatic cholangial drainage, and the biotransformed metabolites profiles over a 120-min period in serum and bile were analyzed. In serum unconjugated 3 alpha-hydroxy-7,12-dioxo-cholanoic acid (3 alpha-OH-7,12-OXO) acid was markedly increased after the injection of dehydrocholic acid, and reached about 80 microM within 30 min. On the other hand, conjugated 3 alpha, 7 alpha-dihydroxy-12-oxo-cholanoic acid (3 alpha,7 alpha-OH-12-OXO) was consecutively increased with lag time to an elevation of 3 alpha-OH-7,12-OXO. In bile, the major constituent of metabolites was conjugated 3 alpha,7 alpha-OH-12-OXO (more than 90% of the excreted metabolites), while conjugated 3 alpha-OH-7,12-OXO was detected as minor constituent. Therefore, between bile and serum, there was great difference in biotransformed metabolites profiles. The mechanism of elevation of unconjugated 3 alpha-OH-7,12-OXO in serum remains obscure, but this new finding raised a question as to whether organ-reducing 3-keto group of dehydrocholic acid is restricted to the liver.
将1克脱氢胆酸静脉注射到两名经皮经肝胆管引流患者体内，并分析了血清和胆汁中120分钟内生物转化代谢物的概况。在血清中，注射脱氢胆酸后未结合的3α-羟基-7,12-二氧代胆酸（3α-OH-7,12-OXO）明显增加，并在30分钟内达到约80微摩尔。另一方面，结合的3α,7α-二羟基-12-氧代胆酸（3α,7α-OH-12-OXO）随着3α-OH-7,12-OXO升高的延迟时间而持续增加。在胆汁中，代谢物的主要成分是结合的3α,7α-OH-12-OXO（超过排泄代谢物的90%），而结合的3α-OH-7,12-OXO作为次要成分被检测到。因此，胆汁和血清之间生物转化代谢物概况存在很大差异。血清中未结合的3α-OH-7,12-OXO升高的机制仍不清楚，但这一新发现引发了一个问题，即脱氢胆酸的3-酮基还原是否仅限于肝脏。
