IIS Aragón, Zaragoza, Spain; Department of Microbiology, Hospital Universitario Miguel Servet, Zaragoza, Spain.
Department of Microbiology, School of Medicine, University of Sevilla, Sevilla, Spain.
Photodiagnosis Photodyn Ther. 2018 Mar;21:211-216. doi: 10.1016/j.pdpdt.2017.11.012. Epub 2017 Nov 28.
Antimicrobial photodynamic therapy (aPDT) could constitute an alternative therapy to antibiotics especially against superficial infections caused by bacteria involved in multidrug resistance processes. The aim of this study is to compare the efficacy of aPDT using the photosensitizer rose bengal (RB), combined or uncombined with gentamicin (GN), against Staphylococcus aureus. Different concentrations of RB (ranging from 0.03 to 64 μg/ml) were added to S. aureus in water suspensions or forming biofilms in the absence or presence of GN (1-40 μg/ml) and the samples were irradiated (18 or 37 J/cm). The number of viable bacteria was quantified by counting colony-forming units. RB-aPDT shows significant photoactivity. The combination of GN and RB-aPDT exerts a synergistic bactericidal effect against planktonic S. aureus. On the other hand, a synergistic effect is observed only when the maximum concentration tested of RB and GN was used in biofilm. According to these result the use of RB-aPDT alone or in combination with GN could be implemented against S. aureus.
抗菌光动力疗法（aPDT）可能是一种对抗生素的替代疗法，特别是针对由涉及多药耐药过程的细菌引起的浅表感染。本研究的目的是比较使用光敏剂孟加拉玫瑰红（RB）联合或不联合庆大霉素（GN）的 aPDT 对金黄色葡萄球菌的疗效。不同浓度的 RB（0.03 至 64μg/ml ）被添加到金黄色葡萄球菌的水悬浮液或生物膜中，在不存在或存在 GN（1-40μg/ml）的情况下进行照射（18 或 37 J/cm）。通过计数集落形成单位来定量活细菌的数量。RB-aPDT 显示出显著的光活性。GN 和 RB-aPDT 的组合对浮游金黄色葡萄球菌具有协同杀菌作用。另一方面，仅当在生物膜中使用 RB 和 GN 的测试最大浓度时，才观察到协同作用。根据这些结果，单独使用 RB-aPDT 或与 GN 联合使用都可以针对金黄色葡萄球菌实施。
