Vladescu Alina, Vranceanu Diana M, Kulesza Slawek, Ivanov Alexey N, Bramowicz Mirosław, Fedonnikov Alexander S, Braic Mariana, Norkin Igor A, Koptyug Andrey, Kurtukova Maria O, Dinu Mihaela, Pana Iulian, Surmeneva Maria A, Surmenev Roman A, Cotrut Cosmin M
National Institute for Optoelectronics, Department for Advanced Surface Processing and Analysis by Vacuum Technologies, 409 Atomistilor St., Magurele, RO77125, Romania.
National Research Tomsk Polytechnic University, Lenin Avenue 43, Tomsk, 634050, Russia.
Sci Rep. 2017 Dec 1;7(1):16819. doi: 10.1038/s41598-017-16985-z.
Properties of the hydroxyapatite obtained by electrochemical assisted deposition (ED) are dependent on several factors including deposition temperature, electrolyte pH and concentrations, applied potential. All of these factors directly influence the morphology, stoichiometry, crystallinity, electrochemical behaviour, and particularly the coating thickness. Coating structure together with surface micro- and nano-scale topography significantly influence early stages of the implant bio-integration. The aim of this study is to analyse the effect of pH modification on the morphology, corrosion behaviour and in vitro bioactivity and in vivo biocompatibility of hydroxyapatite prepared by ED on the additively manufactured Ti64 samples. The coatings prepared in the electrolytes with pH = 6 have predominantly needle like morphology with the dimensions in the nanometric scale (30 nm). Samples coated at pH = 6 demonstrated higher protection efficiency against the corrosive attack as compared to the ones coated at pH = 5 (93% against 89%). The in vitro bioactivity results indicated that both coatings have a greater capacity of biomineralization, compared to the uncoated Ti64. Somehow, the coating deposited at pH = 6 exhibited good corrosion behaviour and high biomineralization ability. In vivo subcutaneous implantation of the coated samples into the white rats for up to 21 days with following histological studies showed no serious inflammatory process.
通过电化学辅助沉积（ED）获得的羟基磷灰石的性能取决于几个因素，包括沉积温度、电解质的pH值和浓度、施加的电势。所有这些因素都直接影响其形态、化学计量、结晶度、电化学行为，尤其是涂层厚度。涂层结构以及表面微观和纳米尺度的形貌显著影响植入物生物整合的早期阶段。本研究的目的是分析pH值改变对通过ED在增材制造的Ti64样品上制备的羟基磷灰石的形态、腐蚀行为、体外生物活性和体内生物相容性的影响。在pH = 6的电解质中制备的涂层主要呈针状形态，尺寸在纳米尺度（约30 nm）。与在pH = 5下涂层的样品相比，在pH = 6下涂层的样品对腐蚀攻击表现出更高的保护效率（约93%对89%）。体外生物活性结果表明，与未涂层的Ti64相比，两种涂层都具有更大的生物矿化能力。不知何故，在pH = 6下沉积的涂层表现出良好的腐蚀行为和高生物矿化能力。将涂层样品皮下植入白色大鼠体内长达21天，随后进行组织学研究，结果显示没有严重的炎症过程。
