Degrelle Severine A, Fournier Thierry
Faculté de Pharmacie de Paris, INSERM, UMR-S1139, Paris, 75006, France.
Université Paris Descartes, Sorbonne Paris Cité, Paris, 75006, France.
Methods Mol Biol. 2018;1710:165-171. doi: 10.1007/978-1-4939-7498-6_13.
In human placenta, the multinucleated syncytiotrophoblast (ST) allows all the exchanges between the maternal and fetal circulation and is also the site of placental hormonal functions. Absence or disturbances of ST formation are associated with a defect or pathologies of pregnancy such as preeclampsia (PE) and intrauterine growth retardation (IUGR). All along pregnancy, the ST is regenerated by fusion of underlying mononucleated villous cytotrophoblasts (VCT). The protocol described here provides details on how GATA3 or TWIST1 immunostaining and analysis can be used to easily assess the in vitro differentiation of human placental cytotrophoblast.
在人胎盘中，多核的合体滋养层细胞（ST）介导母血与胎儿循环之间的所有物质交换，也是胎盘激素功能的发生部位。ST形成的缺失或紊乱与诸如子痫前期（PE）和宫内生长受限（IUGR）等妊娠缺陷或病理状况相关。在整个孕期，ST通过其下方的单核绒毛细胞滋养层细胞（VCT）融合而再生。本文所述方案详细介绍了如何利用GATA3或TWIST1免疫染色及分析来轻松评估人胎盘细胞滋养层细胞的体外分化。
