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二氧化铈纳米颗粒的摄取及其对人胎盘原代滋养层细胞活力、分化和功能的影响

Uptake of Cerium Dioxide Nanoparticles and Impact on Viability, Differentiation and Functions of Primary Trophoblast Cells from Human Placenta.

作者信息

Nedder Margaux, Boland Sonja, Devineau Stéphanie, Zerrad-Saadi Amal, Rogozarski Jasmina, Lai-Kuen René, Baya Ibtissem, Guibourdenche Jean, Vibert Francoise, Chissey Audrey, Gil Sophie, Coumoul Xavier, Fournier Thierry, Ferecatu Ioana

机构信息

Faculté de Pharmacie de Paris, Université de Paris, INSERM UMR-S 1139, 3PHM, F-75006 Paris, France.

BFA, Université de Paris, UMR 8251, CNRS, F-75013 Paris, France.

出版信息

Nanomaterials (Basel). 2020 Jul 3;10(7):1309. doi: 10.3390/nano10071309.

DOI:10.3390/nano10071309
PMID:32635405
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7407216/
Abstract

The human placenta is at the interface between maternal and fetal circulations, and is crucial for fetal development. The nanoparticles of cerium dioxide (CeO NPs) from air pollution are an unevaluated risk during pregnancy. Assessing the consequences of placenta exposure to CeO NPs could contribute to a better understanding of NPs' effect on the development and functions of the placenta and pregnancy outcome. We used primary villous cytotrophoblasts purified from term human placenta, with a wide range of CeO NPs concentrations (0.1-101 μg/cm) and exposure time (24-72 h), to assess trophoblast uptake, toxicity and impact on trophoblast differentiation and endocrine function. We have shown the capacity of both cytotrophoblasts and syncytiotrophoblasts to internalize CeO NPs. CeO NPs affected trophoblast metabolic activity in a dose and time dependency, induced caspase activation and a LDH release in the absence of oxidative stress. CeO NPs decreased the fusion capacity of cytotrophoblasts to form a syncytiotrophoblast and disturbed secretion of the pregnancy hormones hCG, hPL, PlGF, P4 and E2, in accordance with NPs concentration. This is the first study on the impact of CeO NPs using human primary trophoblasts that decrypts their toxicity and impact on placental formation and functions.

摘要

人类胎盘处于母体和胎儿循环的界面，对胎儿发育至关重要。空气污染中的二氧化铈纳米颗粒（CeO NPs）在孕期是一种未经评估的风险。评估胎盘暴露于CeO NPs的后果有助于更好地理解纳米颗粒对胎盘发育、功能及妊娠结局的影响。我们使用从足月人胎盘中纯化的原代绒毛细胞滋养层细胞，采用广泛的CeO NPs浓度（0.1 - 101μg/cm）和暴露时间（24 - 72小时），来评估滋养层细胞对其的摄取、毒性以及对滋养层细胞分化和内分泌功能的影响。我们已证明细胞滋养层细胞和合胞体滋养层细胞均有内化CeO NPs的能力。CeO NPs在无氧化应激的情况下，以剂量和时间依赖性方式影响滋养层细胞的代谢活性，诱导半胱天冬酶激活和乳酸脱氢酶释放。CeO NPs降低了细胞滋养层细胞形成合胞体滋养层的融合能力，并根据纳米颗粒浓度干扰了妊娠激素hCG、hPL、PlGF、P4和E2的分泌。这是第一项使用人原代滋养层细胞研究CeO NPs影响的研究，该研究揭示了其毒性以及对胎盘形成和功能的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/69901dfd6587/nanomaterials-10-01309-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/1ee56667689e/nanomaterials-10-01309-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/fca9738989a4/nanomaterials-10-01309-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/eb417a1a3c5c/nanomaterials-10-01309-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/bbb20610a4b6/nanomaterials-10-01309-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/d2f8c88ce3c7/nanomaterials-10-01309-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/882805f8d8c1/nanomaterials-10-01309-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/69901dfd6587/nanomaterials-10-01309-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/1ee56667689e/nanomaterials-10-01309-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/fca9738989a4/nanomaterials-10-01309-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/eb417a1a3c5c/nanomaterials-10-01309-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/bbb20610a4b6/nanomaterials-10-01309-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/d2f8c88ce3c7/nanomaterials-10-01309-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/882805f8d8c1/nanomaterials-10-01309-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2b/7407216/69901dfd6587/nanomaterials-10-01309-g007.jpg

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