School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, China.
School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, China; Shandong Provincial Engineering Centre on Environmental Science and Technology, 17923 Jingshi Road, Jinan 250061, China.
Bioresour Technol. 2018 Feb;250:449-456. doi: 10.1016/j.biortech.2017.11.067. Epub 2017 Nov 23.
Salinity stress has been verified to be a successful approach to enhance lipid production in high-starch marine algae, and salinity-induced carbon flow switching has been proposed as an algal response specific to brackish water. With the aim of testing this assumption, Chlorella sorokiniana SDEC-18, a low-starch freshwater alga, was grown in BG11 medium with NaCl addition at various concentrations (0, 2, 5, 10, 20, and 30 g/L). The results showed that salinity stress promoted carbon redistribution and starch conversion to lipid. The most desirable lipid productivity of 19.66 mg/L·d occurred in the medium with 20 g/L NaCl, about 2.16 times as high as that in the BG11 medium control. Moreover, microalgae with salinity stress were able to produce biodiesel with a more suitable cloud point, due to a decrease in the saturated fatty acid content. This therefore confirms that low-starch freshwater microalgae can also carry out salinity-induced carbon flow switching.
盐胁迫已被证实是提高高淀粉海洋藻类油脂产量的有效方法，而盐诱导的碳流转换被认为是一种针对咸水的藻类响应。为了验证这一假设，本研究以低淀粉淡水藻小球藻 SDEC-18 为研究对象，在 BG11 培养基中添加不同浓度（0、2、5、10、20 和 30 g/L）的 NaCl 进行培养。结果表明，盐胁迫促进了碳的再分配和淀粉向油脂的转化。在 20 g/L NaCl 培养基中，油脂的最高比生产率为 19.66 mg/L·d，约为 BG11 培养基对照的 2.16 倍。此外，由于饱和脂肪酸含量的降低，具有盐胁迫的微藻能够生产出具有更适宜浊点的生物柴油。因此，这证实了低淀粉淡水微藻也可以进行盐诱导的碳流转换。
