Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, 02142, USA.
Nat Rev Genet. 2018 Feb;19(2):67-80. doi: 10.1038/nrg.2017.97. Epub 2017 Dec 4.
Exciting new technologies are often self-limiting in their rollout, as access to state-of-the-art instrumentation or the need for years of hands-on experience, for better or worse, ensures slow adoption by the community. CRISPR technology, however, presents the opposite dilemma, where the simplicity of the system enabled the parallel development of many applications, improvements and derivatives, and new users are now presented with an almost paralyzing abundance of choices. This Review intends to guide users through the process of applying CRISPR technology to their biological problems of interest, especially in the context of discovering gene function at scale.
激动人心的新技术在推出时往往受到自身的限制，因为获得最先进的仪器或多年的实践经验，无论好坏，都确保了社区的缓慢采用。然而，CRISPR 技术带来了相反的困境，该系统的简单性使得许多应用、改进和衍生产品能够并行开发，现在新用户面临着几乎令人瘫痪的大量选择。本文旨在指导用户将 CRISPR 技术应用于他们感兴趣的生物学问题，特别是在大规模发现基因功能的背景下。
