Vitak Taras Y, Wasser Solomon P, Nevo Eviatar, Sybirna Nataliya O
Department of Evolutionary and Environmental Biology, Faculty of Natural Science, University of Haifa, Mount Carmel, Haifa, Israel.
Department of Evolutionary and Environmental Biology, Faculty of Natural Science, University of Haifa, Mount Carmel, Haifa, Israel; Institute of Evolution, University of Haifa, Mount Carmel, Haifa, Israel.
Int J Med Mushrooms. 2017;19(8):697-708. doi: 10.1615/IntJMedMushrooms.2017021305.
Excessive glucose concentrations in blood and cells promote the intensification of auto-oxidation. This is one of the mechanisms through which free radicals form in hyperglycemia. As a result of hyperglycemia, oxidative stress develops and lipid peroxidation (LPO) is enhanced. Erythrocytes are particularly susceptible to reactive oxygen species and LPO, which can violate cell functions. This article describes the analysis of the influence of mycelia from the medicinal mushrooms Agaricus brasiliensis and Ganoderma lucidum on the enzymatic link of the antioxidant system in rat erythrocytes under streptozotocin-induced diabetes mellitus. Oxidative stress was strengthened in red blood cells of diabetic rats, as evidenced by decreased activity of antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, and by increased amounts of thiobarbituric acid-positive products, which are markers of LPO. Administration of A. brasiliensis and G. lucidum submerged cultivated mycelial powder to animals with streptozotocin-induced diabetes restored superoxide dismutase, catalase, and glutathione peroxidase activity and reduced the amounts of thiobarbituric acid-positive products to control values, but did not affect the activity of glutathione reductase.
血液和细胞中过高的葡萄糖浓度会促进自身氧化加剧。这是高血糖状态下自由基形成的机制之一。高血糖会导致氧化应激的产生，并增强脂质过氧化作用（LPO）。红细胞尤其容易受到活性氧和LPO的影响，这会破坏细胞功能。本文描述了巴西蘑菇和灵芝的菌丝体对链脲佐菌素诱导的糖尿病大鼠红细胞抗氧化系统酶促环节的影响分析。糖尿病大鼠红细胞中的氧化应激增强，这表现为超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶等抗氧化酶活性降低，以及作为LPO标志物的硫代巴比妥酸阳性产物含量增加。给链脲佐菌素诱导的糖尿病动物投喂巴西蘑菇和灵芝深层培养菌丝体粉末后，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性得以恢复，硫代巴比妥酸阳性产物的含量降低至对照值，但对谷胱甘肽还原酶的活性没有影响。
