Statkiewicz Matgorzata, Matuszewska Anna, Jaszek Magdalena, Janusz Grzegorz, Osinska Monika, Sulej Justyna, Stefaniuk Dawid, Mikula Michat, Ostrowski Jerzy
Department of Genetics, Maria Sktodowska-Curie Memorial Cancer Centre and Institute of Oncology, Warsaw, Poland.
Department of Biochemistry, Maria Curie-Sktodowska University, Lublin, Poland.
Int J Med Mushrooms. 2017;19(7):619-628. doi: 10.1615/IntJMedMushrooms.2017021241.
Three bioactive fractions isolated from Cerrena unicolor cultures-crude endopolysaccharide (c-EPS), laccase, and a subfraction of low-molecular weight secondary metabolites-were used to determine potential cytotoxic effects on the mouse melanoma B16-F10 cell line (American Type Culture Collection CRL-6475). The results obtained prove that all examined fractions exhibited activity against the investigated tumor cells. In addition, an evident immunomodulatory effect of the c-EPS fraction was observed. Our results show that the levels of 2 cytokines (tumor necrosis factor-a and chemokine ligand 2) in mouse inner medullary collecting duct mIMCD-3 cells (American Type Culture Collection CRL-2123) stimulated by c-EPS were significantly higher. A lipopolysaccharide model was used at the same concentration (10 μg/mL) as a positive control.
从单色蜡蘑培养物中分离出的三种生物活性组分——粗胞内多糖（c-EPS）、漆酶和低分子量次生代谢物亚组分——用于测定对小鼠黑色素瘤B16-F10细胞系（美国典型培养物保藏中心CRL-6475）的潜在细胞毒性作用。所得结果证明，所有检测的组分均对所研究的肿瘤细胞表现出活性。此外，还观察到c-EPS组分具有明显的免疫调节作用。我们的结果表明，c-EPS刺激的小鼠内髓集合管mIMCD-3细胞（美国典型培养物保藏中心CRL-2123）中2种细胞因子（肿瘤坏死因子-α和趋化因子配体2）的水平显著更高。使用相同浓度（10 μg/mL）的脂多糖模型作为阳性对照。
