Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
Department of Virology and Immunology, Maria Curie-Skłodowska University, Lublin, Poland.
Sci Rep. 2019 Feb 13;9(1):1975. doi: 10.1038/s41598-018-37947-z.
The aim of this study was to investigate the anticancer and antioxidant activities of low molecular weight subfractions isolated from secondary metabolites produced by the wood degrading fungus Cerrena unicolor. Human colon cancer cells (stage I) HT-29 and human normal colon epithelial cells CCD 841 CoTr were used in the research. The present study demonstrated that the low molecular weight subfractions exhibited inhibitory activity towards human colon cancer cells HT-29 at a concentration range of 25-200 μg/mL. All 6 subfractions inhibited proliferation of cells down to 47.5-9.2% at the highest concentrations in a dose-dependent manner. The most desired activity was exhibited by subfractions S, 3, 4, and 5, as the proliferation of HT-29 cells was inhibited to the greatest extent (16.5, 47.5, 42.7, and 26.1% of the control, respectively), while the effect on CCD 841 CoTr cells was the mildest (inhibition to 54.4, 71.4, 79.4, and 53.4%, compared to the control, respectively). The microscopic observation revealed that all extracts induced programmed cell death, i.e. apoptosis (up to 44.4% (subfraction 6) towards HT-29 and less than 20% (most fractions) towards CCD 841 CoTr), with no or a significantly low level of necrosis in both cell lines at the same time.
本研究旨在探讨从木材降解真菌密粘褶菌产生的次生代谢产物中分离得到的低分子量亚组分的抗癌和抗氧化活性。研究中使用了人结肠癌细胞（I 期）HT-29 和人正常结肠上皮细胞 CCD 841 CoTr。本研究表明，低分子量亚组分在 25-200μg/mL 的浓度范围内对人结肠癌细胞 HT-29 表现出抑制活性。所有 6 个亚组分均以剂量依赖性方式抑制细胞增殖，最高浓度下抑制率达 47.5-92%。亚组分 S、3、4 和 5 的活性最强，HT-29 细胞的增殖受到最大程度的抑制（分别为对照的 16.5%、47.5%、42.7%和 26.1%），而对 CCD 841 CoTr 细胞的影响最温和（分别抑制 54.4%、71.4%、79.4%和 53.4%）。显微镜观察显示，所有提取物均诱导程序性细胞死亡，即凋亡（对 HT-29 细胞高达 44.4%（亚组分 6），而对 CCD 841 CoTr 细胞则低于 20%（大多数亚组分）），同时在两种细胞系中均无或仅有低水平的坏死。
