School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300350, China.
Nanoscale. 2017 Dec 14;9(48):19367-19373. doi: 10.1039/c7nr07337b.
Chirality plays essential roles in life systems such that l-amino acids (LAAs) are predominantly found as the building units of protein for organisms. The presence of the d-enantiomer (DAA) has been found to be specifically relevant to gastric cancer. We herein construct a luminescent DNA/silver nanocluster based biosensing system to achieve rapid and specific detection of DAAs. As a proof of application, we detected DAAs in saliva samples from patients with gastric cancer, and the test results exhibited excellent specificity. Our detection system has the following major advantages: (i) the detection is rapid, being completed in less than 1 hour; (ii) the limit of detection falls in the effective range of DAA concentrations of gastric cancer at an early stage, indicating that our method is potentially suitable for early diagnosis of gastric cancer; (iii) the non-invasive sampling manner provides an adaptable system for point-of-care testing (POCT); and (iv) the system does not require any massive instruments or expensive reagents, which enables POCT as well.
手性在生命体系中起着至关重要的作用，因此 l-氨基酸（LAAs）主要作为生物体蛋白质的构建单元。已经发现 d-对映体（DAA）的存在与胃癌特别相关。我们在此构建了一个基于发光 DNA/银纳米簇的生物传感系统，以实现对 DAA 的快速和特异性检测。作为应用的证明，我们检测了胃癌患者唾液样本中的 DAA，测试结果表现出优异的特异性。我们的检测系统具有以下主要优点：（i）检测速度快，不到 1 小时即可完成；（ii）检测限处于胃癌早期 DAA 浓度的有效范围内，表明我们的方法可能适用于胃癌的早期诊断；（iii）非侵入性采样方式为即时检测（POCT）提供了一个适应性强的系统；（iv）该系统不需要任何大型仪器或昂贵的试剂，这也使得 POCT 成为可能。
