Solanki Rohit, Dhaka S S, Ratwan Poonam, Singh Mohit, Kumar Manoj
Department of Animal Genetics and Breeding, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India.
Trop Anim Health Prod. 2025 Jun 25;57(6):286. doi: 10.1007/s11250-025-04539-9.
Pig promises distinct qualities like early maturity, high fecundity, produces piglets between eight to twelve in each farrowing and high feed conversion efficiency that make piggery a low-cost profitable enterprise. The objective of this study was to evaluate the genetic diversity in Large White Yorkshire pigs based on variations in Insulin like growth factor binding protein 2 (IGFBP2) gene which is involved in regulation of embryonic development and association of variants with economic traits. Blood samples were collected from 100 pigs and polymorphism was explored in IGFBP2 gene using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique. A 245 bp fragment in intron 2 of IGFBP2 gene was amplified using PCR. Amplified PCR products of IGFBP2 gene were digested using MspI restriction enzymes. In this study, polymorphic patterns were revealed for the IGFBP2 gene. Two types of genotypes i.e. CC (55 and 190 bp) and CT (55, 190 and 245 bp) were observed for the targeted loci of intron 2 of IGFBP2 gene. The CC genotype was found to have higher genotype frequency as 0.88. Frequencies of 'C' and 'T' alleles were 0.94 and 0.06 in Large White Yorkshire pigs. Results of the Chi-square test showed that the targeted locus of IGFBP2 gene was in Hardy-Weinberg equilibrium (p < 0.05) in the screened pig population. CT genotyped pigs farrowed one more piglet than CC and litter weight at birth was also one kilogram more in CT as compared to the homozygous CC genotyped pigs. A total of 13 samples of intron 2 region of IGFBP2 gene were sequenced and on comparison of sequencing results with reference sequence of Duroc cross (BV727778), a single nucleotide polymorphism (SNP) i.e. T278C was found and there was change in nucleotide at one position i.e. A171G (Transition). In conclusion, CT genotypes of the IGFBP2 gene may be selected further for propagation through breeding programmes in Large White Yorkshire pigs for higher litter size and litter weight at birth.
猪具有早熟、高繁殖力等显著特性，每窝产仔8至12头，且饲料转化率高，这使得养猪业成为低成本盈利的产业。本研究的目的是基于参与胚胎发育调控的胰岛素样生长因子结合蛋白2（IGFBP2）基因的变异，评估大白约克夏猪的遗传多样性，以及变异与经济性状的关联。从100头猪采集血液样本，使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术探究IGFBP2基因的多态性。利用PCR扩增IGFBP2基因第2内含子中的一个245 bp片段。IGFBP2基因的扩增PCR产物用MspI限制性酶消化。在本研究中，揭示了IGFBP2基因的多态模式。在IGFBP2基因第2内含子的目标位点观察到两种基因型，即CC（55和190 bp）和CT（55、190和245 bp）。发现CC基因型具有较高的基因型频率，为0.88。大白约克夏猪中“C”和“T”等位基因的频率分别为0.94和0.06。卡方检验结果表明，在筛选的猪群中，IGFBP2基因的目标位点处于哈迪-温伯格平衡（p < 0.05）。CT基因型的猪比CC基因型的猪每窝多产一头仔猪，且出生时窝重也比纯合CC基因型的猪重1千克。对IGFBP2基因第2内含子区域的13个样本进行了测序，将测序结果与杜洛克杂交（BV727778）的参考序列进行比较，发现一个单核苷酸多态性（SNP）即T278C，并且在一个位置的核苷酸发生了变化，即A171G（转换）。总之，对于大白约克夏猪，IGFBP2基因的CT基因型可进一步通过育种计划选择用于繁殖，以获得更高的产仔数和出生时的窝重。
