Rehani Shweta, Raj Naresh, Jeergal Prabhakar, Sharma Mohit, Bishen Kundendu Arya, Nagpal Ruchi
Department of Oral Pathology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, India.
Department of Oral Pathology, Government College of Dentistry, Indore, Madhya Pradesh, India.
J Cytol. 2021 Oct-Dec;38(4):225-230. doi: 10.4103/JOC.JOC_44_21. Epub 2021 Nov 22.
Occupational exposure to petrol derivatives possesses an increased risk of various cancers including that of the oral mucosa. Scientific studies have shown the correlation of micronuclei assay (MN) with the cytogenotoxic changes in petrol station attendants. However, very few have reported the use of MN assay as a promising tool for assessing the impact of smoking in these workers.
To explore the cytogenotoxic damage in exfoliated buccal cells obtained from petrol station attendants and control subjects using the MN assay along with additional effects due to smoking.
The study comprised 60 males who were divided into Group I-IV with each having 15 subjects. These subjects were categorized as exposed smokers, exposed nonsmokers, unexposed smoker group, and unexposed nonsmokers. The MN and additional nuclear abnormalities (karyorrhexis [KH], binucleation [BN], pyknosis [P], and karyolysis [KL]) were calculated in PAP-stained slides.
Statistically higher mean frequencies of overall nuclear anomalies were observed in petrol pump workers in comparison with the control group. Petrol pump smokers carry the highest nuclear anomalies followed by non-exposed smokers than exposed non-smokers and the count was the least among unexposed non-smoker workers.
The present study indicated that the petrol pump workers are under higher cytogenotoxic damage. Also, smoking added to the frequency of damage. Thus, MN and other nuclear anomalies are in-vitro reliable biomarker assays available and should be routinely employed as a screening tool in their periodic medical evaluation.
职业性接触汽油衍生物会增加患各种癌症的风险，包括口腔黏膜癌。科学研究表明，微核试验（MN）与加油站工作人员的细胞遗传毒性变化相关。然而，很少有研究报道将MN试验作为评估这些工人吸烟影响的一种有前景的工具。
使用MN试验探索从加油站工作人员和对照受试者获得的脱落颊细胞中的细胞遗传毒性损伤，以及吸烟带来的额外影响。
该研究包括60名男性，分为I-IV组，每组15名受试者。这些受试者被分类为暴露吸烟者、暴露不吸烟者、未暴露吸烟者组和未暴露不吸烟者。在巴氏染色的载玻片上计算MN和其他核异常（核固缩[KH]、双核化[BN]、核浓缩[P]和核溶解[KL]）。
与对照组相比，在加油站工作人员中观察到总体核异常的平均频率在统计学上更高。加油站吸烟者的核异常最多，其次是未暴露吸烟者，然后是暴露不吸烟者，未暴露不吸烟者的核异常计数最少。
本研究表明，加油站工作人员受到更高的细胞遗传毒性损伤。此外，吸烟增加了损伤频率。因此，MN和其他核异常是可靠的体外生物标志物检测方法，应在他们的定期医学评估中作为常规筛查工具使用。
