Antitumor Effects of Ethanol Extracts from in H Tumor-bearing Mice.

BACKGROUND

Research the antitumor effects of ethanol extracts from in H tumor-bearing mice. At the fist-stage of the experiments, the research team took MTT method to measure the antitumor activity , then selected the most inhibitory tumor cell strain as the test object of antitumor activity , established three models of a solid tumor H liver cancer, ascites tumor, and immunodeficiency in male mice. From inflammatory factor, liver toxicity, antioxidant index to observe antitumor activity of ethanol extracts from .

MATERIALS AND METHODS

Hundred and twenty ICR male mice were used to establish three models of a solid tumor H liver cancer, ascites tumor, and immunodeficiency in male mice and models group of a solid tumor H liver cancer randomly divided into six groupshe normal control group, the model control group, the positive group (cyclophosphamide), the sample treated group (high - 1.300 g/kg, medium - 0.750 g/kg, low - 0.373 g/kg). The animals were sacrificed 15d after oral administration and tumors were taken out for the tumor weights and antitumor rates. Blood in eyeball was collected for the determination of aspartate transaminase, alanine transaminase, malondialdehyde (MDA), superoxide dismutase (SOD), interleukin (IL)-2, and tumor necrosis factor (TNF)-α in serum. Sections of tumor issue were prepared, and morphological changes in tumor tissue cells were observed using hematoxylin and eosin staining technique.

RESULTS

The results showed that ethanol extracts from have a certain inhibitory effect on the digestive tumor cells. In solid tumor model, the inhibitory rate is up to 68.84% of the high dose of treated group from , and could improve the immune organ index, decrease the concentration of TNF-α and IL-2 in serum. In ascites tumor model, could slow down weight gain in mice and prolong the survival time; in immunodeficiency model, could improve the serum TNF-α and, IL-2 levels, increase SOD activity, and reduce MDA content, so as to achieve antitumor effect.

CONCLUSIONS

Ethanol extracts from have obvious antitumor activity and can improve a tumor-burdened mice inflammation factors, improve the survival quality of H tumor mice, and enhance immunity and antitumor activity.

SUMMARY

Ethanol extracts from have obvious antitumor activity without obviously liver damageThe experiment results prompt that the antitumor activity probably caused by decreasing the inflammatory factors, improve the survival quality, enhance the abnormal cytokines and scavenging free radicals, and raising autoimmune function in H tumor bearing mice. H. rhomboidea: Hyptis rhomboidea; AST: Aspartate transaminase; ALT: Alanine transaminase; MDA: Malondialdehyde; SOD: Superoxide dismutase; IL-2: Interleukin 2; TNF-α: Tumor Necrosis Factor-α; CTX: Cyclophosphamide.