慢性乙型肝炎肝硬化和肝细胞癌患者肝细胞中IP-10、p53和Foxp3的表达

IP-10, p53, and Foxp3 Expression in Hepatocytes of Chronic Hepatitis B Patients with Cirrhosis and Hepatocellular Carcinoma.

作者信息

Shahera Umme, Munshi Saifullah, Jahan Munira, Nessa Afzalun, Alam Shahinul, Tabassum Shahina

机构信息

Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahabag, Dhaka, Bangladesh.

Department of Hepatology Bangabandhu Sheikh Mujib Medical University, Shahabag, Dhaka, Bangladesh.

出版信息

Euroasian J Hepatogastroenterol. 2016 Jul-Dec;6(2):149-153. doi: 10.5005/jp-journals-10018-1188. Epub 2016 Dec 1.

DOI:10.5005/jp-journals-10018-1188

PMID:29201748

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5578584/

Abstract

AIM

Elucidating differences in gene expression may be useful in understanding the molecular pathogenesis and for developing specific markers for the outcome of hepatitis B virus (HBV) infection. In the present study, expressions of host gene interferon gamma-inducible protein (IP-10), p53, and Foxp3 were studied in hepatocytes of patients with chronic HBV infection to determine a possible link between selected host gene expression and the outcome of HBV infection.

MATERIALS AND METHODS

The study was conducted in 60 patients with chronic HBV infection and they were divided into four groups: HBV-positive cirrhosis (n = 15), HBV-negative cirrhosis (n = 15), HBV-positive hepatocellular carcinoma (HCC) (n = 15) and HBV-negative HCC (n = 15). Total messenger ribonucleic acid (mRNA) extraction was done followed by complementary deoxyribonucleic acid (cDNA) synthesis, and finally gene expression was performed using real-time polymerase chain reaction (PCR) technique.

RESULTS

IP-10 and p53 gene expressions were lower in HBV-positive cirrhosis, and Foxp3 gene expression was upregulated in HBV-positive cirrhosis in comparison to HBV-negative cirrhosis. The expressions of all the three genes were upregulated among HBV-positive HCC in comparison to HBV-negative HCC. The expression of IP-10, p53, and Foxp3 genes was upregulated in HBV-positive HCC in comparison to HBV-positive cirrhosis.

CONCLUSION

This study indicates that there are variations in the expression of the selected genes among cirrhosis and HCC patients with or without HBV. All the three selected genes were more or less upregulated in HBV-positive HCC patients, but only Foxp3 expression was upregulated in HBV-positive cirrhosis. These three particular genes may have a role in the molecular pathogenesis and clinical outcome of HBV-positive cirrhosis and HCC patients. These aspects need further evaluation by studies with larger numbers of cirrhosis and HCC patients.

HOW TO CITE THIS ARTICLE

Shahera U, Munshi S, Jahan M, Nessa A, Alam S, Tabassum S. IP-10, p53, and Foxp3 Expression in Hepatocytes of Chronic Hepatitis B Patients with Cirrhosis and Hepatocellular Carcinoma. Euroasian J Hepato-Gastroenterol 2016;6(2):149-153.

摘要

目的

阐明基因表达差异可能有助于理解分子发病机制，并为开发乙型肝炎病毒（HBV）感染结局的特异性标志物提供帮助。在本研究中，对慢性HBV感染患者的肝细胞中宿主基因干扰素γ诱导蛋白（IP - 10）、p53和Foxp3的表达进行了研究，以确定所选宿主基因表达与HBV感染结局之间的可能联系。

材料与方法

对60例慢性HBV感染患者进行了研究，并将他们分为四组：HBV阳性肝硬化组（n = 15）、HBV阴性肝硬化组（n = 15）、HBV阳性肝细胞癌（HCC）组（n = 15）和HBV阴性HCC组（n = 15）。先进行总信使核糖核酸（mRNA）提取，随后进行互补脱氧核糖核酸（cDNA）合成，最后使用实时聚合酶链反应（PCR）技术进行基因表达检测。

结果

与HBV阴性肝硬化相比，IP - 10和p53基因在HBV阳性肝硬化中的表达较低，而Foxp3基因在HBV阳性肝硬化中上调。与HBV阴性HCC相比，所有这三个基因在HBV阳性HCC中的表达均上调。与HBV阳性肝硬化相比，IP - 10、p53和Foxp3基因在HBV阳性HCC中的表达上调。

结论

本研究表明，在有或无HBV的肝硬化和HCC患者中，所选基因的表达存在差异。在HBV阳性HCC患者中，所有这三个所选基因或多或少都上调，但仅Foxp3表达在HBV阳性肝硬化中上调。这三个特定基因可能在HBV阳性肝硬化和HCC患者的分子发病机制及临床结局中起作用。这些方面需要通过对更多肝硬化和HCC患者的研究进行进一步评估。