31P-NMR 证明紫红酵母经丁香霉素处理的细胞中存在细胞质酸化和磷酸盐外排现象。

31P-NMR evidence for cytoplasmic acidification and phosphate extrusion in syringomycin-treated cells of Rhodotorula pilimanae.

作者信息

Reidl H H, Grover T A, Takemoto J Y

机构信息

Department of Biology, Utah State University, Logan 84322-0300.

出版信息

Biochim Biophys Acta. 1989 Mar 6;1010(3):325-9. doi: 10.1016/0167-4889(89)90056-6.

DOI:10.1016/0167-4889(89)90056-6

PMID:2920179

Abstract

31P-NMR spectroscopy was used to investigate the effects of the phytotoxin, syringomycin, on phosphate metabolism and intracellular pH changes in the yeast Rhodotorula pilimanae. Syringomycin, at levels between 20 and 60 units per 10(8) cells, caused a cellular efflux of orthophosphate. At 40 and 60 unit per 10(8) cells, the efflux was accompanied by a decrease in polyphosphate and an acidification of the cytoplasm. At low temperatures (5 degrees C) and with 75 units per 10(8) cells, these effects were more rapid and pronounced. The efflux of phosphate was confirmed by chemically assaying extracellular phosphate after syringomycin treatment.

摘要

采用³¹P-核磁共振光谱法研究了植物毒素丁香霉素对酵母皮膜红酵母中磷酸盐代谢及细胞内pH变化的影响。每10⁸个细胞中丁香霉素含量在20至60单位之间时，会导致正磷酸盐从细胞中流出。每10⁸个细胞中丁香霉素含量为40和60单位时，流出伴随着多聚磷酸盐减少和细胞质酸化。在低温（5℃）且每10⁸个细胞含有75单位丁香霉素时，这些影响更为迅速且明显。经丁香霉素处理后，通过化学分析细胞外磷酸盐证实了磷酸盐的流出。