Exploring the Leaves Hemostatic and Wound-Healing Potential.

The present paper investigated the efficiency of on hemostatic and wound healing activities. leaf extracts were evaluated for their antibacterial and antioxidant effects as well as their flavonoid and polyphenol content. The hydroethanolic extract (EtOH-HOE), showing the most potent antibacterial and antioxidant activities , thanks to its flavonoid and polyphenol richness, was selected for hemostatic and wound healing evaluation. Twenty-four rats completing full-thickness wounds were split into four groups. The wounds were topically treated with saline solution, glycerol, "CICAFLORA," and EtOH-HOE (50 L/mm) until day 11. The wound healing effect was assessed by macroscopic, histological, and biochemical parameters. Rats treated with EtOH-HOE showed fast wound closure (92.39%) compared to the control animals (60.91%) on the 11th day of wounding ( < 0.01). Histopathological and biochemical explorations showed full epidermal regeneration and an improvement of the hydroxyproline content in the EtOH-HOE treated rats. Analysis of fatty acids and sterols by GC-MS showed the presence of unsaturated fatty acids and a high concentration of lupeol known for their involvement in reepithelialization. These results prove the efficiency of EtOH-HOE in wound healing and supported its traditional use.