Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
ISP, INRA, Université François Rabelais de Tours, 37380, Nouzilly, France.
Vet Res. 2017 Dec 4;48(1):85. doi: 10.1186/s13567-017-0493-7.
Gut microbiota is of considerable importance for each host. Despite this, germ-free animals can be obtained and raised to sexual maturity and consequences of the presence or absence of gut microbiota on gene expression of the host remain uncharacterised. In this study, we performed an unbiased study of protein expression in the caecum of germ-free and colonised chickens. The major difference between these two groups was in the expression of immunoglobulins which were essentially absent in the germ-free chickens. Microbiota also caused a minor decrease in the expression of focal adhesion and extracellular matrix proteins and an increase in the expression of argininosuccinate synthase ASS1, redox potential sensing, fermentative metabolic processes and detoxification systems represented by sulfotransferases SULT1C3 or SULT1E1. Since we also analysed expression in the caecum of E. coli Nissle and E. faecium DSM7134 mono-associated chickens, we concluded that at least immunoglobulin expression and expression of cystathionine synthase (CBS) was dependent on microbiota composition with E. coli Nissle stimulating more immunoglobulin and PIGR expression and E. faecium DSM7134 stimulating more CBS expression. Gut microbiota and its composition therefore affected protein expression in the chicken caecum though except for immunoglobulin production, the remaining differences were unexpectedly low.
肠道微生物群对每个宿主都非常重要。尽管如此，仍然可以获得无菌动物并将其饲养至性成熟，并且肠道微生物群的存在与否对宿主基因表达的影响尚未确定。在这项研究中，我们对无菌和定植鸡的盲肠中的蛋白质表达进行了无偏研究。这两组之间的主要区别在于免疫球蛋白的表达，无菌鸡的免疫球蛋白基本上不存在。微生物群还导致黏着斑和细胞外基质蛋白的表达略有下降，而精氨酸琥珀酸合成酶 ASS1、氧化还原电势感应、发酵代谢过程和解毒系统（由磺基转移酶 SULT1C3 或 SULT1E1 代表）的表达增加。由于我们还分析了大肠杆菌 Nissle 和屎肠球菌 DSM7134 单定植鸡盲肠中的表达，我们得出结论，至少免疫球蛋白表达和胱硫醚合酶 (CBS) 的表达依赖于微生物群落组成，大肠杆菌 Nissle 刺激更多的免疫球蛋白和 PIGR 表达，屎肠球菌 DSM7134 刺激更多的 CBS 表达。因此，肠道微生物群及其组成影响了鸡盲肠中的蛋白质表达，尽管除了免疫球蛋白产生外，其余差异出人意料地低。
