Schindeler Aaron, Mills Rebecca J, Bobyn Justin D, Little David G
Orthopedic Research and Biotechnology Unit, The Children's Hospital at Westmead, Locked Bag 4001, Westmead, Sydney, New South Wales, 2145, Australia.
Discipline of Pediatrics and Child Health, Faculty of Medicine, University of Sydney, Sydney, Australia.
J Orthop Res. 2018 Mar;36(3):832-840. doi: 10.1002/jor.23824. Epub 2017 Dec 19.
In this review, we broadly define and discuss the preclinical rodent models that are used for orthopedics and bone tissue engineering. These range from implantation models typically used for biocompatibility testing and high-throughput drug screening, through to fracture and critical defect models used to model bone healing and severe orthopedic injuries. As well as highlighting the key methods papers describing these techniques, we provide additional commentary based on our substantive practical experience with animal surgery and in vivo experimental design. This review also briefly touches upon the descriptive and functional outcome measures and power calculations that are necessary for an informative study. Obtaining informative and relevant research outcomes can be very dependent on the model used, and we hope this evaluation of common models will serve as a primer for new researchers looking to undertake preclinical bone studies. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:832-840, 2018.
在本综述中，我们广泛定义并讨论了用于骨科和骨组织工程的临床前啮齿动物模型。这些模型涵盖范围广泛，从通常用于生物相容性测试和高通量药物筛选的植入模型，到用于模拟骨愈合和严重骨科损伤的骨折和关键缺损模型。除了重点介绍描述这些技术的关键方法论文外，我们还根据我们在动物手术和体内实验设计方面的丰富实践经验提供了额外的评论。本综述还简要涉及了信息丰富的研究所必需的描述性和功能性结果测量以及功效计算。获得信息丰富且相关的研究结果很大程度上取决于所使用的模型，我们希望对常见模型的这种评估能为希望开展临床前骨研究的新研究人员提供入门指导。© 2017骨科研究协会。由威利期刊公司出版。《矫形外科学研究》36:832 - 840，2018年。
